Lecture 12: Biotechnology + Human Diseases

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Last updated 2:17 AM on 10/15/26
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18 Terms

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TaqI

sticky ends

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Hae III

blunt ends

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RNA-dependent DNA polymerase

synthesizes DNA strand

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ribonuclease H

degrades RNA strand of RNA-DNA hybrid

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DNA-dependent DNA polymerase

synthesizes 2nd DNA strand to create cDNA

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chemical synthesis uses…

activated monomer deoxyribonucleoside 3’-phosphoramidite

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PCR - polymerase chain reaction

  1. denature DNA

  2. anneal primers

  3. extend primers


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gel electrophoresis

negative phosphodiester backbone, shorter fragment travels farther

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southern blot

DNA

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northern blot

RNA

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western blot

protein

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microarray

contains thousands of immobilized DNA sequences

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RNA sequencing

sequence entire RNA component

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ELISA

detect and quantify proteins

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radioimmunoassay

radioactivity

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proteomics

mass spectrometry

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SDS

denatures proteins

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2-mercaptoethanol

reduces disulfide bonds