Gram stain steps

Gram stain purpose

the purpose of a gram stain is to differentiate bacteria into two separate groups: gram positive and gram negative based on the differences in their cell wall structures, specifically the thickness of their peptidoglycan layers

Heat fixing purpose

kills vegetative cells and lets the bacteria adhere to the slide, preserving the cell’s structure and makes sure the bacteria does not get rinsed off

Crystal violet

60s, enters and stains all bacterial cells walls and stains them a deep purple color

Iodine

60s,