Lab 2 Enterobacter Tests

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Last updated 11:45 PM on 2/25/26
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11 Terms

1
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Lysine iron agar ingredients

  1. Lysine

  2. Glucose

  3. Brom cresol purple

  4. Sodium thiosulfate

  5. Iron

LIA tube

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<p>Lysine results </p>

Lysine results

  1. Purple = positive for lysine decarboxylase

    1. Need acidity to turn on reaction

  2. Yellow = negative

  3. Black = H2S

  4. Burgandy = Lysine deamination


3
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MIO Tube tests for

  1. Motility

  2. Indole

  3. Ornithine decarboxylase


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<p>Ornithine decarboxylase</p>

Ornithine decarboxylase

  1. Asses bottom of tube

    1. Positive = Purple

    2. Negative = Yellow


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Phenylalanine deaminase function

  1. Converts phenylalanine to ammonia and phenylpyruvic acid


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Phenylalanine deaminase addition

Ferric chloride

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<p>Phenylalanine deaminase interpretation </p>

Phenylalanine deaminase interpretation

  1. Green = positive

  2. Yellow = negative


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Voges-Proskauer Reagents

  1. VP Reagent 1 = alpha naphthol

  2. VP Reagent 2 = KOH


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<p>Voges-Proskauer interpretation</p>

Voges-Proskauer interpretation

Red/pink = Positive

Yellow/ colorless = Negative

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ONPG Function

  1. Bacteria needs both B-galactosidase and B-permease

  2. Ortho-nitrophenyl acts as surrogate to glucose


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<p>ONPG interpretation</p>

ONPG interpretation

Yellow = Positive

Colorless = Negative