Lab 2 Enterobacter Tests

Lysine iron agar ingredients

1. Lysine

2. Glucose

3. Brom cresol purple

4. Sodium thiosulfate

5. Iron

LIA tube

Lysine results

1. Purple = positive for lysine decarboxylase

   1. Need acidity to turn on reaction

2. Yellow = negative

3. Black = H2S

4. Burgandy = Lysine deamination

MIO Tube tests for

1. Motility

2. Indole

3. Ornithine decarboxylase

Ornithine decarboxylase

1. Asses bottom of tube

   1. Positive = Purple

   2. Negative = Yellow

Phenylalanine deaminase function

1. Converts phenylalanine to ammonia and phenylpyruvic acid

Phenylalanine deaminase addition

Ferric chloride

Phenylalanine deaminase interpretation

1. Green = positive

2. Yellow = negative

Voges-Proskauer Reagents

1. VP Reagent 1 = alpha naphthol

2. VP Reagent 2 = KOH

Voges-Proskauer interpretation

Red/pink = Positive

Yellow/ colorless = Negative

ONPG Function

1. Bacteria needs both B-galactosidase and B-permease

2. Ortho-nitrophenyl acts as surrogate to glucose

ONPG interpretation

Yellow = Positive

Colorless = Negative