Module 7 Biotechnology

Genetic engineering is _____.

the transfer of a gene from one organism to another

In recombinant DNA technology, how are genes of interest "cut" from DNA?

Using restriction enzymes

In gel electrophoresis, if there are DNA segments that are 5,000, 10,000, and 15,000 base pairs long, which segment will be found closest to the negative end of the chamber?

15,000

In recombinant DNA technology, how is a DNA molecule amplified?

polymerase chain reaction

Which of the following is not commonly used by genetic engineers?

a sperm cell from a donor father

Plasmids are used for genetic engineering because _____.

they carry genes, can be manipulated, and move between bacterial cells

Plasmids used by genetic engineers will have a(n) _____.

All are correct

DNA polymerase _____.

makes copies of DNA

A scientist attempts to insert a plasmid with a foreign gene into bacteria. Not all the bacteria will take the plasmid in. How can the scientist select transformed bacteria and know that they have been transformed?

Give the plasmid a gene for antibiotic resistance, grow the bacteria in agar with an antibiotic. The surviving colonies will have the plasmid.

The process of transformation involves _____.

shocking bacteria and mixing them with recombinant plasmids