FORMALIN ETHYL ACETATE CONCENTRATION TECHNIQUE
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28 Terms
Formalin Ethyl Acetate Concentration Technique
What does FEACT stands for?
Formalin Ethyl Acetate Concentration Technique (FEACT)
It is a concentration produce that employs the principle of sedimentation
Sedimentation
What principle does FEACT uses?
10% formalin, Ether/ Ethyl acetate
Reagents used for FEACT
lower specific gravity
FEACT uses solution of _________ rather than parasitic organisms
Can be used for helminth eggs and protozoan cysts
Sediment can be stored for long period of time
Advantages of FEACT
10 parts
How many parts of distilled water needed for FEACT
One part
How many parts of stool needs for FEACT
half size of tumb
Size of stool needed
2 layers for gauze
Strain the fecal emulsion though how many layers of gauze?
Supernatant
What is needed to throw away after centrifuging the emulsion?
Clear
Supernatant should be colored ______ after several washing
3
How many washings and centrifuge are usually sufficient for the supernatant to be clear?
10 ml
Amount of 10% formalin when mixing with fecal sediment
10 - 30 minutes
standing time for fixation to take place
Protozoan cysts
what does 10% formalin stopped from being distorted?
3 ml of ethyl acetate
what is being added to formalinized specimen?
2 minutes
time when centrifuging FEACT
pipette
What is being used to transfer sediment to a glass slide?
small drop
how many drops of 2% iodine solution to stain the protozoan cysts?
2% iodine solution
What is being drop to stain protozoan cysts?
Protozoan cysts
what is being stained by a small drop of 2% iodine solution?
Ether - Bulk debris / debris plug - Formalin - Sediment
Layers of FEACT (from top to bottom)
Sediment
What is the 4th layer of FEACT?
Species and Stage
For interpretation of results, if parasite eggs, larvae and adults are seen, what does it include?
No Intestinal Parasite Seen (NIPS)
Negative Result should be reported as?
Health and Safety Benefits, Performance Benefits
Advantages of Mini Parasep
F3cal Parasitic Concentrator
Other term for Mini Parasep