spectrophotometer

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35 Terms

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wavelength

The spectrophotometer technique is to measure light intensity as a function of _______.

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prism

In the spectrophotometer, a _______ (or) grating is used to split the incident beam into different wavelengths.

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wavelengths

In the spectrophotometer, a prism (or) grating is used to split the incident beam into different _______.

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1 to 2nm

By suitable mechanisms, waves of specific wavelengths can be manipulated to fall on the test solution. The range of the wavelengths of the incident light can be as low as _______.

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wavelength

The spectrophotometer is useful for measuring the absorption spectrum of a compound, that is, the absorption of light by a solution at each _______.

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energy

. A table and cheap radiant _______ source

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electrical heating

Materials that can be excited to high energy states by a high voltage electric discharge (or) by _______ serve as excellent radiant energy sources.

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monochromator

A _______, to break the polychromatic radiation into component wavelength (or) bands of wavelengths.

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wavelengths

: A monochromator resolves polychromatic radiation into its individual _______ and isolates these wavelengths into very narrow bands.

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narrow

A monochromator resolves polychromatic radiation into its individual wavelengths and isolates these wavelengths into very _______ bands.

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prism

A _______ disperses polychromatic light from the source into its constituent wavelengths by virtue of its ability to reflect different wavelengths to a different extent.

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cornu

: Two types of Prisms are usually employed in commercial instruments. Namely, 600 _______ quartz prism and 300 Littrow Prism.

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Littrow

Two types of Prisms are usually employed in commercial instruments. Namely, 600 Cornu quartz prism and 300 _______ Prism.

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Gratings

_______ are often used in the monochromators of spectrophotometers operating ultraviolet, visible and infrared regions.

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ultraviolet

Gratings are often used in the monochromators of spectrophotometers operating _______, visible and infrared regions.

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visible

Gratings are often used in the monochromators of spectrophotometers operating ultraviolet, _______ and infrared regions.

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infrared

Gratings are often used in the monochromators of spectrophotometers operating ultraviolet, visible and _______ regions.

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Transport

_______ vessels (cuvettes), to hold the sample

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CUVETTES

Samples to be studied in the ultraviolet (or) visible region are usually glasses (or) solutions and are put in cells known as “_______ ”

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glass

Cuvettes meant for the visible region are made up of either ordinary _______ (or) sometimes Quartz.

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detector

A Photosensitive _______ and an associated readout system

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photoelectric

Most detectors depend on the _______ effect. The current is then proportional to the light intensity and therefore a measure of it.

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intensity


: Most detectors depend on the photoelectric effect. The current is then proportional to the light _______ and therefore a measure of it.

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transmitter

Radiation detectors generate electronic signals which are proportional to the _______ light.

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recorders

This is accomplished by using amplifiers, Ammeters, Potentiometers and Potentiometric _______.

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concentration

Detection of _______ of substances

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impurities

Detection of _______

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elucidation

Structure _______ of organic compounds

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freshwater

Monitoring dissolved oxygen content in _______ and marine ecosystems

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oxygen

Monitoring dissolved _______ content in freshwater and marine ecosystems

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proteins

Characterization of _______

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functional

Detection of _______ groups

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analysis

Respiratory gas _______ in hospitals

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weight

Molecular _______ determination of compounds

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identify

The visible and UV spectrophotometer may be used to _______ classes of compounds in both the pure state and in biological preparations.