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Photosynthesis
process where plants convert light energy (from the sun) to chemical energy
-process produces glucose and oxygen
In photosynthesis what is oxidized and what is reduced (water)
Water is oxidized to form O2
In photosynthesis what is oxidized and what is reduced (CO2)
Co2 is reduced to form glucose
where does photosynthesis take place
takes place in the chloroplast
-light dependent reactions in thylakoid membrane and Calvin cycle in the stroma
where does O2 take place
comes from H2O split during light dependent reactions
2 pathways in photosynthesis
light dependent reactions
Calvin cycle
Light dependent reactions
produce ATP and NADPH
splits water and releases oxygen
functions of light dependent reactions
-absorb light using pigments such as chlorophyll
-excite electrons with light energy
-split water, releasing oxygen
-establish a proton gradient across the thylakoid membrane
-produce ATP and NADPH
ATP
supplies energy for the reactions in the Calvin cycle including regeneration of RuBP
-supplies energy
NADPH
supplies high energy electrons and reducing power needing to convert carbon containing molecules into G3P
-supplies reducing power
Calvin cycle
uses carbon dioxide, ATP, and NADPH to produce G3P, a three-carbon sugar that can be used to make carbohydrates
Stroma
where Calvin cycle occurs and carbohydrates are produced
Thylakoid membranes
contains chlorophyll, photosystems, and electron transport chain needed for the light-dependent reaction
-help establish a proton gradient that is used to produce ATP
Grana
stacks of thylakoids that provide an organized membrane structure for the light dependent reactions
Carboxylation (carbon fixation)
CO2 is attached to RuBP by the enzyme rubisco, forming (3-PGA)
Reduction
ATP and NADPH provides the energy and reducing power needed to convert 3-PGA into G3P
Regeneration
remaning G3P molecules use ATP to regenerate the starting molecule, RuBP so the cycle can continue
G3P
three-carbon sugar produced during the Calvin cycle
-used to make larger carbohydrates
What is light
form of electromagnetic radiation that travels in waves and packets on energy called photons
what molecules convert light into chemical energy
pigments, specifically chlorophyll
Chlorophyll
pigment that absorbs light energy during photosynthesis
Chlorophyll when absorbs light
chlorophyll gets excited when it absorbs a photon
-absorption spectrum: graph showing how much light energy absorbs at various wavelengths
-action spectrum: a graph showing the rate of photosynthesis driven by different wavelengths of light
main pigments of chlorophyll
chlorophyll alpha and chlorophyll beta
why do plants have accessory pigments
they provide photoprotection by absorbing and safely dissipating excess light energy that could otherwise damage the plant
what is an antenna system
a cluster of hundreds of pigment molecules embedded in the thylakoid membrane that harvests light energy and funnel it toward a central reaction center
how electrons are transferred through the antenna systems
one pigment absorbs a photon, passes to it’s neighbor until the energy reaches the center chlorophyll
noncyclic electron transport
electrons flow from photosystem 2, and photosystem 1 to reduce NADP+ into NADPH, producing ATP, NADPH, and oxygen as a byproduct
-2 photosystems involved
-NADPH and ATP are synthesized via photophosphorylation
cyclic photophosphorylation
excited electrons returned to the photosystem they came from. This pathway produces ATP but does not produce NADPH or release oxygen through water splitting
noncyclic photophosphorylation
electrons move through the photosynthesis electron transport system and ultimately help reduce NADP to NADPH. Water replaces the electrons lost from the photosystem, and oxygen is released. ATP is also produced
How is a plant in the dark affected by photosynthesis
light- dependent reactions cannot operate normally because they pigments cannot capture the light energy needed to excite electrons
Reception
signal (ligand) binds to a receptor which is activated
Transduction
Signal is transmitted to the interior of the cell by a signal transduction pathway
Response
cell responds, by activating an enzyme or turning on transcription of a gene
Termination
signal stopped so response does not continue unnecessarily
Paracrine signaling
targets another cell with molecules in between, close but not connected
-short distance
Synoptic signaling
neuron communicating with a target cell across a specialized junction known as a synapse
Endocrine signaling
long distance signaling where cell released hormones into fluid that travel to other cells away
-travels through blood cells typically
Autocrine signaling
cell secretes molecule that binds to own receptor and causes a response, stimulating itself
-same cell
Photosystem
group of pigments and proteins organized in the thylakoid membrane
accessory pigments
absorb light and transfer energy toward the reaction center
reaction center
contains a special chlorophyll pair that uses the transferred energy to excite elections
Growth factor
protein that stimulates local cells to grow, divide, mature
-typically involved in paracrine signaling
intracellular signaling
inside the cell
intercelular signaling
outside the cell
receptors
proteins where signal molecules can bind
-located on the plasma membrane
hydrophilic signaling molecules
cannot pass through the plasma membrane so they bind to the membrane receptors
-facilitated diffusion or active transport
hydrophobic signaling molecules
can pass through the lipid bilayer and bind to intracellular receptors
-simple diffusion
polar and non polar receptors
both receptors go through shape change upon bonding with the ligand
G protein coupled receptors
cell surface transmembrane receptor that works with the help of a G protein, which binds energy-rich GTP
When ligand bonds to intercellular side the GPCR changes shape and activates protein which detaches and binds to an enzyme to trigger the next step
Receptor Kinases
activate signaling pathways involving the addition of phosphate groups to proteins. they can regulate growth and cell division
Ion channel receptors
control the movement of ions across the membrane in response to a signal, changing the cells electrical or chemical conditions
Ligand-gated ion channels
membrane receptors containing a region that act as a gate when the receptor changes shape
second messenger
small non-protein, water-soluble molecule or ion that relays a signal to a cells interior in response to a signaling molecule bound by a signal receptor protein (the first messenger)
how small signaling molecules like adrenaline cause big responses
protein pathways involve a cascade of enzymes activation leading to the activation of molecules in the next step
single amplified in a GPCR signaling pathway
-can activate multiple G protein molecules
-can produce large second messenger molecules like cAMP
-each protein kinase an phosphorylate and activate multiple copies of the next protein kinase in a series
Signaling errors and cancer
mutations that modify proteins in cell signaling can lead to excessive cell growth, division, and survival even in the absence of growth factor which is a hallmark of cancer
Role of Ras in cancer
protein relays a signal from growth factor receptors to a protein kinase cascade. mutations that make Ras permanently active causes continuous unregulated cell division even when no growth factor is present, driving cancer development
cell signaling
process which cells send, receive, and respond to messages
ligand
signaling molecule that carries the message
responding cell
cell that receives the message and produces a response
contact dependent signaling
when cells communicate through direct physical contact
transmembrane proteins
extend through the cell membrane and can interact with molecules on neighboring cells. these interactions allow cells to recognize each other and exchange information
Gap junctions
allow small molecules and ions pass through them for communication
cell structure and cell function related
cells structure helps it do its job
cell
basic unit of life
tissue
group of similar cells working together to do a job
organ
contains different tissues working together to perform a function
epidermis
outer skin layer
dermis
supports epidermis underneath
keratinocytes
makes keratin for protection
melanocytes
make pigment
fibroblasts
produces collagen for strength
cytoskeleton
supports the cell and helps it maintain its shape
microtubules
transport materials
help maintain cell shape, transport materials inside cells, move chromosomes during cell division, and support cilia and flagella
microfilaments
help with movement
-made of actin. help cells move
intermediate filaments
provide strength and stability. help cells resist tearing easily
dynamic instability
when microtubules grow and shrink. allows cells to change shape, move, and prepare for cell division
kinesin and dynein
motor proteins that carry materials along microtubules in different directions
cilia
move materials across cell surfaces
flagella
helps cell move
Cell adhesion molecules (CAM’s)
help cells attach to other cells or the extracellular matrix. they keep cells connected and organized into tissues
Adherens junctions
connect neighboring cells and help maintain shape
desmosomes
hold cells together strongly
hemidesmosomes
anchor cells to the underlying layer
tight junctions
prevent substances from passing between cells
cadherins
connect cells to other cells
intergrins
connect cells to the extracellular matrix
cell division
process where one cell makes more cells
growth, cell replacement, healing, reproduction
reproductive signal
tells the cell when to divide
DNA replication
copies the genetic material
segregation
ensures each new cell gets a full DNA copy
cytoplasmic division
splits the cells contents into two
binary fission
simple type of cell division used by bacteria
binary fission (1)
DNA attaches to the inner cell membrane
binary fission (2)
DNA replication begins at the origin replication
binary fission (3)
The new DNA attaches to the membrane
binary fission (4)
cell elongates, separating the DNA copies
binary fission (5)
new cell walls and membranes form in the middle
binary fission (6)
cell splits into two identical cells
prokaryotic cell division
have circular DNA in the cytoplasm and divide through binary fission