BIOL 231 DNA Extraction Lab

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8 Terms

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Polymorphic DNA

chromosomal regions that vary widely from individual to individual

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DNA fingerprint

found by examining multiple polymorphic DNA regions in one person

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DNA fingerprint uses

paternity/maternity, kinship, human remains identification, criminal offenders, genetic basis of various diseases

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Who/Where/When developed

Dr. Alex Jeffreys, UK, 1984 (used in 1987 murder case)

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polymerase chain reaction (PCR) use

amplifies small amounts of polymorphic DNA prior to analysis

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what enzyme copies a DNA template in PCR

Taq polymerase

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Taq polymerase characteristics

purified from bacteria from hot springs, stable at high temperatures

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