HEMOGLOBIN

IN HEMOGLOBIN STRUCTURE HOW MANY GLOBIN CHAINS ARE PRESENT?

4

IN HEMOGLOBIN STRUCTURE HOW MANY PROTOPORPHYRIN IX ARE PRESENT?

4

IN HEMOGLOBIN STRUCTURE HOW MANY FERROUS IRON ARE PRESENT?

4

IN HEMOGLOBIN STRUCTURE 1 GRAM OF HGB CAN CARRY ____ IRON

3.47 MG

IN HEMOGLOBIN STRUCTURE HOW MANY 2-3 DPG/BPG ARE PRESENT?

1

THIS IS THE OXYGEN-BINDING PROTEIN IN RBC

HEMOGLOBIN

WHAT ARE THE MAIN CYTOPLASMIC COMPONENT OF RBC

1/3 OF RBC WEIGHT AND 34G/DL PER RBC

PRODUCTION PERCENT OF HEMOGLOBIN IN NUCLEATED STAGES OF ERYTHROPOIESIS

65%

PRODUCTION PERCENT OF HEMOGLOBIN IN RETICULOCYTE STAGE

35%

1 GRAM OF HGB IS EQUAL TO HOW MUCH OF O2

1.34 ML

HEME IS ALSO KNOWN AS?

FERRIPROTOPORPHYRIN IX

HEME BELONGS TO THE CLASS OF PIGMENTS KNOWN AS?

PORPHYRINS

1 HEME CAN BIND TO?

ONE O2

THIS OCCURS FROM PRONORMOBLAST STAGE TO RETICULOCYTE STAGE

GLOBIN SYNTHESIS

THIS IS HEMOGLOBIN BOUND TO OXYGEN, BRIGHT RED IN COLOR, AND FOUND IN ARTERIAL BLOOD

OXYHEMOGLOBIN (HBO2)

HEMOGLOBIN CONFORMATION OF OXYHEMOGLOBIN

RELAXED (R) STATE

HEMOGLOBIN NOT BOUND TO OXYGEN, DARK RED IN COLOR, FOUND IN VENOUS BLOOD

DEOXYHEMOGLOBIN

HEMOGLOBIN CONFORMATION OF DEOXYHEMOGLOBIN

TENSED (T) STATE

WHAT ARE DYSHEMOGLOBINS

METHEMOGLOBIN, SULFHEMOGLOBIN, CARBOXYHEMOGLOBIN

METHEMOGLOBIN IS ALSO KNOWN AS?

HEMIGLOBIN, FERRIHEMOGLOBIN

THIS IS HEMOGLOBIN WITH FERRIC IRON (FE3+)

HEMIN

DYSHEMOGLOBIN THAT ARE NOT ABLE TO BIND TO OXYGEN

METHEMOGLOBIN

COLOR OF BLOOD WITH METHEMOGLOBIN

CHOCOLATE BROWN

>30% OF METHEMOGLOBIN CAUSES?

HYPOXIA

TRUE OR FALSE. METHEMOGLOBIN IS IRREVERSIBLE

FALSE

IT IS A STABLE, GREEN-PIGMENT MOLECULE, WHICH IS NOT NORMALLY PRESENT IN THE BODY

SULFHEMOGLOBIN

THIS IS A MIXTURE OF OXIDIZED, AND PARTIALLY DENATURED HEMOGLOBIN

SULFHEMOGLOBIN

COLOR OF BLOOD WITH SULFHEMOGLOBIN

MAUVE-LAVENDER, GREENISH, BLUISH

THIS IS A HEMOGLOBIN WITH FE2+ BOUND TO CO

CARBOXYHEMOGLOBIN

THIS IS THE KNOWN TO BE THE SILENT KILLER (AWOO)

CARBON MONOXIDE

THIS HAS A 200-240X MORE AFFINITY TO HEMOGLOBIN THAN O2

CARBON MONOXIDE

IN WHAT PERCENTAGE CAN CARBOXYHEMOGLOBIN CAUSE HEADACHE AND DIZZINESS

10-15%

IN WHAT PERCENTAGE CAN CARBOXYHEMOGLOBIN CAUSE COMA AND CONVULTIONS

>50%

COLOR OF BLOOD WITH CARBOXYHEMOGLOBIN

CHERRY-RED

REVERSIBLE OR IRREVERSIBLE. CARBOXYHEMOGLOBIN

REVERSIBLE

GLYCOSYLATED HEMOGLOBIN IS ALSO KNOWN AS?

GLYCATED HEMOGLOBIN, GLYCOHEMOGLOBIN

HOW MANY PERCENT IS HBA1C IN CIRCULATION?

4-6%

2 MECHANISMS FOR DEGRADATION OF HEMOGLOBIN

EXTRAVASCULAR AND INTRAVASCULAR CATABOLISM

WITH EXTRAVASCULAR HEMOLYSIS, THE ERYTHROCYTES ARE DEGRADED WITHIN _______

MACROPHAGES

HEMOGLOBIN ARE DISSOCIATED TO?

IRON, GLOBIN, PROTOPORPHYRIN

THIS IS TRANSPORTED TO THE BONE MARROW FOR RECYCLING (TRANSFERRIN)

IRON

THIS IS CATABOLIZED INTO THE LIVER

GLOBIN

THIS IS CATABOLIZED TO BILIRUBIN TO UROBILINOGEN AND IS EXCRETED BY THE KIDNEYS

PROTOPORPHYRIN

WHAT IS THE ALTERNATE PATHWAY FOR DEGRADATION OF HEMOGLOBIN

INTRAVASCULAR CATABOLISM

THIS IS THE REFERENCE METHOD FOR HEMOGLOBIN ASSAY

CYANMETHEMOGLOBIN METHOD

REAGENT FOR CYANMETHEMOGLOBIN METHOD

DRABKIN’S REAGENT

REAGENT FOR CYANMETHEMOGLOBIN METHOD CONSISTS OF?

POTASSIUM FERRICYANIDE, POTASSIUM CYANIDE, NON-IONIC DETERGENT, AND DIHYDROGEN POTASSIUM PHOSPHATE

THIS IMPROVES RBC LYSIS

NON-IONIC DETERGENT

ABSORBANCE OF CYANMETHEMOGLOBIN AT _____ IS ________ TO THE HEMOGLOBIN CONCENTRATION

540NM; DIRECTLY PROPORTIONAL

FOR HEMOGLOBIN MEASUREMENT, SHAKING, WHOLE BLOOD IS SHAKEN IN THE AIR FOR?

15 MINUTES

WHAT TEST/METHOD FOR CARBOXYHEMOGLOBIN

KATAYAMA’S TEST

WHAT METHOD IS USED FOR QUALITATIVE, SIMPLE DIRECT VISION

SPECTROSCOPE

SI UNIT (NM) FOR HBCO IN SPECTROPHOTOMETRIC METHOD

555 nm

SI UNIT (NM) FOR Hi IN SPECTROPHOTOMETRIC METHOD

630 nm

SI UNIT (NM) FOR SHb IN SPECTROPHOTOMETRIC METHOD

630 nm

What test is performed for the examination of Plasma or serum for Fairly’s Pigment

Schumm’s test

A positive results in schumm’s test shows?

Narrow band is seen in green filter paper at 558 nm

GLOBIN CHAINS OF PORTLAND

2 ZETA 2 GAMMA

GLOBIN CHAINS OF GOWER I

2 EPSILON 2 ZETA

GLOBIN CHAINS OF GOWER II

2 ALPHA 2 EPSILON

GLOBIN CHAINS OF HEMOGLOBIN F (HBF)

2 ALPHA 2 GAMMA

GLOBIN CHAINS OF HEMOGLOBIN A1

2 ALPHA 2 BETA

GLOBIN CHAINS OF HEMOGLOBIN A2

2 ALPHA 2 DELTA

PROPORTION IN NEWBORN OF HEMOGLOBIN A1

20%

PROPORTION IN NEWBORN OF HEMOGLOBIN A2

0.5%

PROPORTION IN NEWBORN OF HEMOGLOBIN F

80%

PROPORTION IN ADULTS OF HEMOGLOBIN A1

97%

PROPORTION IN ADULTS OF HEMOGLOBIN F

<1%

PROPORTION IN ADULTS OF HEMOGLOBIN A2

2.5%

WHAT CHAINS ARE PRESENT IN CHROMOSOME 16

ALPHA, ZETA

WHAT CHAINS ARE PRESENT IN CHROMOSOME 11

BETA, DELTA, GAMMA, EPSILON

NUMBER OF AMINO ACIDS. ALPHA

141

NUMBER OF AMINO ACIDS. BETA

146

NUMBER OF AMINO ACIDS. GAMMA A

146 (POSITION 136: ALANINE)

NUMBER OF AMINO ACIDS. GAMMA G

146 (POSITION 136: GLYCINE)

NUMBER OF AMINO ACIDS. DELTA

146

NUMBER OF AMINO ACIDS. EPSILON

UNKNOWN

NUMBER OF AMINO ACIDS. ZETA

141

NUMBER OF AMINO ACIDS. THETA

UNKNOWN

IN OXYHEMOGLOBIN DISSOCIATION CURVE, A SHIFT TO THE LEFT CAUSES AN INCREASED AND DECREASED ON WHAT?

INCREASED O2 AFFINITY AND PH, DECREASED IN PCO2, 2,3-BPG, TEMPERATURE

IN OXYHEMOGLOBIN DISSOCIATION CURVE, A SHIFT TO THE RIGHT CAUSES AN INCREASED AND DECREASED ON WHAT?

INCREASED PCO2, 2,3-BPG, TEMPERATURE, DECREASED O2 AFFINITY AND PH

TO PRODUCE CYANMETHEMOGLOBIN, HEMOGLOBIN IS TURNED TO METHEMOGLOBIN BY __________, AND METHEMOGLOBIN WILL RESULT TO CYANMETHEMOGLOBIN THROUGH ________

POTASSIUM FERRICYANIDE; POTASSIUM CYANIDE

MANY INSTRUMENTS NOW USE ________ TO CONVERT HEMOGLOBIN TO SLS-METHEMOGLOBIN

SODIUM LAURYL SULFATE (SLS)

TWO TEST IN DETERMINING OF FETAL HEMOGLOBIN

ALKALI DENATURATION AND ACID ELUTION TEST

THIS TEST HAS A PRINCIPLE OF HBF RESISTING ALKALI DENATURATION WHILE HBA DOES NOT

ALKALI DENATURATION TEST

THIS TEST HAS A PRINCIPLE OF RESISTING ACID ELUTION, THUS APPEAR AS ISOLATED DARKLY — STAINING CELL AMONG A BACKGROUND OF PALE STAINING GHOST CELLS

ACID ELUTION TEST

NORMAL VALUE OF FETAL HEMOGLOBIN IN ALKALI DENATURATION TEST FOR ADULTS

0.5-8%

NORMAL VALUE OF FETAL HEMOGLOBIN IN ALKALI DENATURATION TEST FOR 1 YEAR OLD BABIES

1%

NORMAL VALUE OF FETAL HEMOGLOBIN IN ACID ELUTION TESTQ

1-5% OF RBC CONTAIN RESIDUAL HBF

Hgb molecules in an alkaline solution have a net ___________ and move toward the anode in an electrophoretic system

negative charge

Electrophoretic mobility greater than HbA at pH ___ are known as __________

8.6; fast hemoglobin

What are fast hemoglobin

Hb Bart’s, Hb H, Hb I

Slowest Hgb

Hgb C

What is the pH of Cellulose Acetate Agar

8.4-8.6

What is the screening procedure for Hgb electrophoresis

Cellulose Acetate Agar

Buffer of Cellulose Acetate Agar

Tris EDTA Boric Acid

Stain for the screening test of Hgb electrophoresis

Ponceau S

What is the pH of Citrate Agar

6.0-6.2

What is the confirmatory test of Hgb electrophoresis

Citrate Agar

Specific Gravity Method is also known as?

Copper sulfate method