Hexokinase
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6 Terms
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Step 1 of Hexokinase
glucose + Mg2+ + ATP → hexokinase → glucose-6-phosphate
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Step 2 of Hexokinase
glucose-6-phosphate + NADP+ → glucose-6-deydrogenase → 6-P-gluconate + NADPH
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How do we measure NADPH
Spectrophotometer at 340 nm
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glucose-6-P-dehydrongenase
Enzyme that is specific and has less interference than peroxidase
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False decreased result
Increased bilirubin due to hemolysis
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This is considered a
reference method (whole blood/ plasma/ serum)
Note 
Flashcards (37)