VT BIOL 1115 CRISPR I

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5 Terms

1
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What is a plasmid?

  • a small circular DNA molecule


2
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What is antibiotic selection in bacterial transformation?

  • uses antibiotic resistance gene to kill off bacteria to make sure only transformed bacteria survive


3
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How are plasmids different from chromosomal DNA? Mention two points.

  • size and base pairs

  • independent replication


4
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What does a promoter do on a plasmid?

  • acts as a green light for transcription

  • binds RNA polymerase


5
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How can plasmids help study gene function?

  • track specific species or populations

  • observe phenotypic changes