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Immunocytochemistry
using anitbodies to label proteins and visualize
Immunohistochemistry
using antibodies to label proteins in tissues/ brain slice
in situ hybridization
using complimentary nucleic acids to bind and tag mRNA
autoreceptors
neurotransmitters present on the presynaptic neuron. regulate activity
Cholinergic neurons
Acetyl CoA and Choline and made into acetylcholine by ChAT enzyme —>ACH is transported and released by vesicle —> ACHe breaks it down into choline and acetic acid
Catecholaminergic
makes dopamine, norepinepherine, or epinepherine. Tyrosine →DOPA →Dopamine→Norepinepherine→Epinepherine. MAO degrades
Serotonergic Neurons
Tryptophan (diet) precursor of serotonin. SSRIs prevent reuptake of serotonin. Degraded by MAO
Amino Acidergic Neurons
Glutamate(excitatory), GABA, Glycine(inhibitory). EAAT take glutamate from synaptic cleft.
Endocannabinoid
Oligodendrocytes
create mylelin
Astrocytes
surround the synaptic gap and release gliotransmitters. Water transport across brain. Maintain blood brain barrier.
Microglia
resident immune cells. macrophages, eat nulclei