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What information can hair evidence give investigators? What kind of evidence is hair evidence?
Information it can give: Hair color, texture, length, and microscopic characteristics; may also provide DNA if the root is present.
Type of evidence: Trace evidence
Evidence classification: Usually class evidence, but DNA testing can potentially make it individual evidence
Parts of the hair shaft (cuticle, medulla, cortex, pigment/melanin granules, etc.).
Cuticle: Outer protective layer; made of overlapping scales.
Cortex: Middle layer; contains melanin/pigment that gives hair its color.
Medulla: Inner core; may be continuous, fragmented, or absent.
Melanin granules: Pigment particles in the cortex that determine hair color.
Medulla patterns found in humans (4)
Continuous: The medulla forms one unbroken line from the root toward the tip of the hair.
Interrupted: The medulla is present but has clear gaps along its length.
Fragmented: The medulla appears as small, separated pieces rather than a continuous line.
Absent: There is no visible medulla in the hair shaft.
Cuticle patterns (imbricate, coronal, spinous-what kinds of animals have these? What do humans have? What do they look like?
Imbricate → Humans
Cuticle scales overlap like shingles on a roof.
This is the most common human cuticle pattern.
Coronal → Small rodents (like mice and rats)
Scales form crown-like rings around the hair shaft.
Looks like stacked crowns/cups.
Spinous → Cats, seals, and some other animals
Scales have pointed, petal-like edges that stick outward.
Looks spiky or thorn-like.
Easy way to remember:
🧑 Humans = Imbricate → overlapping shingles
🐭 Rodents = Coronal → crowns
🐱 Cats = Spinous → spikes
Calculating a medullary index.
Medullary Index: A ratio comparing the diameter of the medulla to the diameter of the entire hair shaft.
Formula: Medullary Index = Medulla diameter ÷ Hair shaft diameter
Example: If the medulla is 0.03 mm and the hair shaft is 0.09 mm:
0.03 ÷ 0.09 = 0.33
Forensics: Humans usually have a medullary index less than ⅓, while many animals have an index greater than ½.
Using a medullary index to determine if a hair is from a human or animal.
Medullary index:
Human hair: Usually less than ⅓
Animal hair: Usually greater than ½
Formula: Medulla diameter ÷ total hair diameter
What are other ways we can tell a human hair apart from an animal hair?
Other differences:
Medulla: Humans often have a thin, fragmented, or absent medulla; animals often have a thicker, more prominent medulla.
Cuticle: Humans have imbricate scales; animals may have coronal or spinous scales.
Pigment: Human pigment is usually more evenly distributed; animal pigment may be more concentrated or patterned.
Root: Human and animal hair roots can have different shapes and appearances
Calculate how many days a hair has grown if you know the length grown.
Human hair grows about 0.5 mm per day.
Formula:
Days = length grown ÷ 0.5 mm/day
Example: If hair grew 10 mm:
10 ÷ 0.5 = 20 days.
Characteristics of hair to determine racial/ethnic background.
Hair characteristics can suggest ancestry, but they cannot reliably identify a person’s race/ethnicity by themselves.
Investigators may examine:
Hair shape/cross-section (round, oval, flattened)
Texture and thickness
Pigment distribution
Degree of curl
Life cycle of hair (anagen, catagen, telogen-what happens in these stages?
Anagen (Growth):
Hair is actively growing.
Root is usually soft, elongated, and club-like with tissue attached.
Hair is firmly attached to the follicle.
Catagen (Transition):
Hair stops actively growing.
Follicle shrinks and the hair begins separating from its blood supply.
Shortest stage.
Telogen (Rest):
Hair is no longer growing and rests in the follicle.
Eventually the hair falls out naturally.
Root is typically hard, dry, and club-shaped.
We find a hair, how can we tell if it was in the anagen stage or telogen stage?
Anagen hair: Root has tissue attached and looks actively growing → usually has a soft, elongated appearance.
Telogen hair: Root is club-shaped, dry, and lacks tissue → indicates the hair naturally shed.
Easy memory: Anagen = attached & growing; Telogen = resting & shedding.
What kind of DNA can you acquire from a hair in the anagen stage? Telogen stage? What does this mean?
Anagen hair: Can contain nuclear DNA because the root still has living cells/tissue.
Nuclear DNA is highly individualizing and can potentially identify a specific person.
Telogen hair: Usually does not contain enough nuclear DNA because the root is no longer living tissue.
It can still contain mitochondrial DNA (mtDNA), which is useful for determining a maternal lineage, but is less specific than nuclear DNA.
How can we use hair to determine if a person has been poisoned with heavy metals like arsenic?
Heavy metals such as arsenic can accumulate in hair as it grows.
Investigators can test sections of the hair for arsenic concentrations.
Because hair grows at a fairly predictable rate, the location of arsenic along the hair shaft can provide clues about when exposure occurred.
This can help investigators determine whether someone was exposed to a heavy metal over time, rather than just at one specific moment.