lab #8: dna extraction

ethanol makes dna

precipitate

bead-beating is a _____ method of lysing cells.

physical/mechanical

the beads are made of

glass or ceramic beads

dna will stick to the silica spin filter until we perform elution with __ buffer.

te buffer

when performing dna electrophoresis the bubbles should be on the side where the samples were loaded or which side of the gel the bubbles are on and ask if the electrodes need to be reversed before running the gel?

false, bubbles form at the positive (+) electrode, opposite the sample-loading wells. If bubbles are on the loading side, the electrodes may be reversed.

the buffer used in gel electrophoresis reduces the resistance of electric current. this prevents the agarose gel from

overheating or melting

bead beating increases the likelihood  of ____ the dna.

shearing (breaking DNA into smaller fragments)

what cation is required for enzymes like DNase and RNase to work?

magnesium (mg²⁺)

what component of TE buffer grabs the ion?

EDTA

taq polymerase is isolated from an organism that can be found in a hot spring in Yellowstone National Park. what domain of life does this thermophilic organism belong to?

bacteria