Y9 - DNA + PROTEINSYNTHESIS + CELLS + MICROSCOPES - BIOLOGY 1

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Last updated 4:42 PM on 10/14/26
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92 Terms

1
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What is the first step when extracting DNA from fruit?

Mash the fruit to break up the tissue.

2
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Why is detergent used during DNA extraction?

It helps break down cell and nuclear membranes.

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Why is salt solution used during DNA extraction?

It helps the DNA separate from other cell components.

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Why is the fruit mixture filtered?

To remove solid pieces of fruit.

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Why is cold ethanol added during DNA extraction?

DNA precipitates out and becomes visible.

6
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What does extracted DNA often look like?

White, cloudy strands or clumps

7
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What is the function of the nucleus?

It contains the genetic material in a eukaryotic cell.

8
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What is the function of the cell membrane?

It controls the movement of substances into and out of the cell.

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What is the function of the cytoplasm?

It is where many chemical reactions take place.

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What is the function of mitochondria?

They are the site of aerobic respiration.

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What is the function of ribosomes?

They are the site of protein synthesis.

12
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What is the function of the cell wall in plant cells?

It supports and strengthens the cell.

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What is the function of chloroplasts?

They are the site of photosynthesis.

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What is the function of the permanent vacuole in plant cells?

It contains cell sap and helps keep the cell rigid.

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What is a eukaryotic cell?

A cell with its DNA enclosed in a nucleus

16
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What is RNA?

A nucleic acid involved in protein synthesis.

17
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What base does RNA use instead of thymine?

Uracil (U).

18
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What sugar is found in DNA?

Deoxyribose.

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What sugar is found in RNA?

Ribose.

20
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How many strands does DNA usually have?

Two strands.

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How many strands does RNA usually have?

One strand

22
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What is a gene?

A section of DNA that codes for a particular protein.

23
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What is a chromosome?

A long DNA molecule carrying many genes.

24
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How is DNA organised in a eukaryotic cell?

DNA is organised into chromosomes in the nucleus; chromosomes contain genes.

25
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Why is DNA important for inheritance?

It carries genetic information that can be passed from parents to offspring.

26
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What is meant by a genome?

The complete set of genetic material in an organism.

27
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Can DNA make copies of itself?

Yes. DNA can replicate to produce copies of its genetic information.

28
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How many chromosome pairs do humans usually have?

23 pairs in most body cells.

29
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What is the relationship between genes and proteins?

Genes contain the code for making particular proteins.

30
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What is the difference between a gene and a chromosome?

A gene is a section of DNA; a chromosome is a long DNA molecule containing many genes.

31
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What is the role of chromosomes?

They package DNA and carry genes.

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What is the role of genetic information in inheritance?

It passes instructions from parents to offspring.

33
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Where is DNA found in a eukaryotic cell?

In the nucleus, organised into chromosomes

34
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What is a mutation?

A change in the DNA base sequence.

35
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How can a mutation affect a protein?

It may change the amino acid sequence and alter the protein's shape or function.

36
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Does every mutation change a protein?

No. Some mutations do not change the protein.

37
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What can happen if a protein's shape changes?

Its function may change or it may stop working properly

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42
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What is magnification?

How many times larger an image is than the actual object.

43
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What is resolution?

The ability to distinguish two close points as separate.

44
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What is the magnification formula?

Magnification = image size Ă· actual size.

45
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How do you calculate actual size?

Actual size = image size Ă· magnification.

46
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How do you calculate image size?

Image size = actual size × magnification.

47
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What units might be used to measure cells?

Micrometres (”m).

48
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How many micrometres are in one millimetre?

1,000 ”m.

49
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What is a light microscope used for?

Viewing cells and larger cell structures.

50
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What is an electron microscope?

A microscope that uses electrons to produce a highly detailed image.

51
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Why can electron microscopes show more detail?

They have a higher resolution than light microscopes.

52
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Why might a stain be added to a microscope slide?

To make structures easier to see.

53
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What does a microscope's scale bar show?

A reference length used to estimate the actual size of structures.

54
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What is the difference between magnification and resolution?

Magnification enlarges an image; resolution determines how much detail can be distinguished

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What is a protein made from?

A chain of amino acids.

56
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How many different amino acids are used to build proteins?

20 different types.

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Where are proteins made in cells?

At ribosomes.

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What is protein synthesis?

The process of using genetic information to build proteins.

59
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What are the two stages of protein synthesis?

Transcription and translation.

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What is transcription?

Making an mRNA copy of a gene from DNA.

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Where does transcription happen?

In the nucleus of a eukaryotic cell.

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What happens to DNA during transcription?

The gene unwinds and its strands separate so a complementary mRNA copy can form.

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What does mRNA stand for?

Messenger ribonucleic acid.

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Why is mRNA needed?

It carries a copy of the genetic code from DNA to a ribosome.

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What happens to mRNA after transcription?

It leaves the nucleus and travels to a ribosome.

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What is translation?

The process of using mRNA instructions to assemble amino acids into a protein.

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Where does translation happen?

At a ribosome.

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What is a codon?

A group of three bases on mRNA.

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What does tRNA do during translation?

It brings the correct amino acids to the ribosome.

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What links amino acids together?

Peptide bonds.

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What is a polypeptide?

A chain of amino acids.

72
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What determines the order of amino acids in a protein?

The sequence of bases in the gene, carried by mRNA.

73
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Why does the amino acid sequence matter?

It affects how a protein folds and functions.

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What is the difference between transcription and translation?

Transcription makes mRNA from DNA; translation uses mRNA to assemble a protein.

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What is the difference between DNA and mRNA?

DNA stores genetic information; mRNA carries a copy of the code to a ribosome.

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Why must DNA bases be read in the correct order?

Their sequence carries the instructions for making proteins.

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What is the role of amino acids in proteins?

They are the building blocks joined to form proteins.

78
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Name examples of proteins.

Enzymes, antibodies, collagen and insulin

79
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What does DNA stand for?

Deoxyribonucleic acid.

80
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What is the main role of DNA?

It carries the genetic code used to make proteins.

81
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What is the shape of a DNA molecule?

A double helix: two strands twisted together.

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How many different bases are found in DNA?

Four.

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Name the four bases in DNA.

Adenine (A), thymine (T), cytosine (C) and guanine (G).

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What are the complementary DNA base-pairing rules?

Adenine pairs with thymine (A–T); cytosine pairs with guanine (C–G).

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What does complementary mean in DNA?

Each base pairs with its specific matching base.

86
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What is a DNA nucleotide?

A DNA building block made of sugar, phosphate and a base.

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What are the monomers of DNA?

Nucleotides.

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What forms the backbone of DNA?

Alternating sugar and phosphate groups.

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What information do DNA bases carry?

The genetic code.

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What connects the two strands of DNA?

Complementary base pairs.

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Which base pairs with adenine in DNA?

Thymine.

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Which base pairs with cytosine in DNA?

Guanine