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DNA
negatively charged molecule found in all living things that serves as the blueprint for which proteins are made
DNA in eukaryotes
nucleus
DNA in prokaryotes
nucleoid
Monomer for DNA
nucleotide
Chargaff’s base pairing rule
Adenine and thymine, guanine and cytosine
Purines
Adenine and guanine
Pyrimidines
Thymine and cytosine
Why is DNA useful?
It can you exactly who was at the scene because every person has a unique genome
Ribosomes
make proteins
Mitochondira
make ATP through cellular respiration
Cell membrane
controls what comes in and out of the cell, maintaining homeostasis
Eukaryote
makes unicellular and multicellular, complex membrane bound organelles
Prokaryote
unicellular, no complex membrane bound organelles
How many chromosomes in a human body cell?
46
What is the purpose of PCR?
It makes millions of copies of DNA so scientists can analyze it even if there is a small amount given
Denaturation
Heat breaks open hydrogen bonds holding each strand of DNA
Annealing
lab made primers bind to gene of interest on the DNA strands. mark the beginning for new DNA strands to be made
Extension
TAQ polymerase adds in free nucleotides to build brand new complementary strands
Restriction Enzymes
cut DNA into recognition sites