MLTS 2060 - Media Charts

BAP, SBA (Sheep Blood Agar)

• primary plate

• enriched media

• differential for hemolysis

CNA (Columbia Colistin Nalidixic Acid)

• selective for gram pos.

• differential for hemolysis

MAC (MacConkey)

• selective for gram neg.

• differential for lactose fermentation (pink = LF, clear = NLF)

*NOTE: enteric pathogens are clear

MAC with Sorbitol

• differential: E. Coli HO157 = clear, Serratia = red

*NOTE: clear doesn’t ferment sorbitol

EMB (Eosin Methylene Blue)

• selective for gram neg. rods

• differential for lactose fermentation

• E. coli = shiny metallic green

CHOC (Chocolate)

• enriched for fastidious organisms

• contains X and V factors

• Neisseria, Haemophilus, Moraxella spp.

MTM (Modified Thayer Martin) (Martin Lewis)

• selective chocolate based for Neisseria

• contains: Nystatin - inhibits yeast; Vancomycin - inhibits GP; Collistin - inhibits GN; Trimethoprim lactate - inhibits proteus

HE / XLD

• selective and differential for salmonella and shigella

• HE = Shigella green, Salmonella black

• XLD = Shigella red, Salmonella black

• non-pathogens = yellow lactose fermenters

TCBS

selective and differential: vibrio cholera = yellow; other coliforms = green

CAMPY BAP

• selective for Campylobacter

• contains: Brucella agar base, 10% sheep RBCs, vancomycin, trimethoprim, poly B, amphotericin B and cephalothin

• incubate at 42℃

CIN (Cefsulodin-Irgasan-Novobiocin)

• selective: Yersinia = pink bullseye (also, Aeromonas)

• contains: sodium deoxycholate, cefsulodin, irgasan, novobiocin

SAB (Sabouraud Dextrose Agar)

fungal media

BE (Bile Esculin)

selective and differential for Bacteroides Fragilis group = black colonies (Gp D strep and Enterococcus are also black)

Reagan-Lowe

• selective for Bordetella pertussis

• transport media

Bordet Gengou

selective for Bordetella pertussis

BYE (Buffered Yeast Extract) (Charcol and Yeast)

selective for legionella

Cysteine Glucose Agar

selective for Francisella Tularenis

LIM Broth (Todd Hewitt)

enrichment broth that suppresses other flora present and enhances Strep agalactiae (Gp B)

L-J (Lowenstein-Jensen) and Middlebrook Medium (Middlebrook 7H10, 7H11)

mycobacterium = acid-fast bacteria (Mycobactrium tuberculosis)

THIO

nutrient broth or agar plate

CDC - ANA Anaerobic Plate (ANA-BAP)

selective for anaerobes

BBA (Brucella Blood Agar)

obligate and facultative anaerobes

BBE (Bacteroides Bile Esculin)

• bile-tolerant Bacteroides spp.

• differential for B. fragilis

LKV or KV (Laked Kanamycin-Vancomycin Blood Agar)

• anaerobic

• supports bacteroides and Prevotella spp.

• prevents other growth

PEA (Phenylethyl Alcohol Agar)

• supports anaerobes

• inhibits enteric gram neg. rods

Chopped Meat Broth

• nutrient medium

• supports all types of bacteria mainly anaerobes

GN and SF Broth

• selective and differential for stool enteric pathogens

• GN = gram neg

• SF = Selenite F broth

Mannitol Salt Agar

• selective for staph

• differential for mannitol fermentation

• S. aureus = yellow; other staph = pink