Homologous Recombination, CRISPR Gene Editing, and SCAR-TRACE Flashcards | Quizlet

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4 Terms

1
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How does the addition of a single nucleotide affect DNA charge?
Adding a single nucleotide introduces a negative phosphate group, affecting the overall charge of the DNA molecule.
2
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What is the purpose of using a DNA size ladder in gel electrophoresis?
A DNA size ladder serves as a reference to determine the size of DNA fragments in the samples being analyzed.
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What is the role of ethidium bromide in DNA visualization?
Ethidium bromide intercalates between DNA nucleotides and fluoresces under UV light, allowing for visualization of DNA in gels.
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What is the outcome of the Meselson-Stahl experiment after three replication cycles?
After three cycles, the DNA shows a mixture of hybrid and light bands, indicating multiple rounds of replication.