Unit 9: Carbohydrate Tests

What media type is TSI?

differential

What media type is Lactose and Sucrose broth?

differential

What media type is MR and VP?

differential

What substrate does TSI sugars have?

Glucose lactose sucrose

What substrate does MR have?

glucose

What substrate does the Iron slant have in TSI?

cytosine theosulfate

What substrate does VP have?

glucose

What enzyme does TSI sugars have

lactase, sucrase, glycolidic

What enzyme does TSI Iron slant have?

cytosine desulfhydrase theosulfate reductase

What product does Lactose and sucrose broths have?

acid CO2

What product does TSI sugars have?

Acid

What product does TSI Iron slant have?

H2S

What product does MR have?

acid

What product does VP have?

alcohol

What indicator does Lactose and Sucrose broth have?

phenol red

What indicator does TSI sugar have?

phenol red

What indicator does TSI Iron slant have?

FeSO4

What indicator does MR have?

Methyl red

What indicator does VP have?

Barr H. reagent

What is positive for Lactose and Sucrose broths?

yellow

What is negative for Lactose and Sucrose broths?

red orange

Which enzymes do the Slant and Butt test?

lactase sucrase, glycolitic

what is positive color for TSI?

yellow acidic

What is negative color for TSI?

red alkaline

What is positive for H2S TSI?

black non-coliform pathogenic

What is positive for MR?

red acid +

What is negative MR?

no red

What is positive for VP?

red ring alcohol +

What is negative for VP?

no red ring

What tool do you use it inoculate slant?

streak surface, stab needle