LPS-induced inflammation in MRC-5 cells

what is MRC-5 cells?

adherent non-cancerous human lung fibroblast cell line

why use MRC-5 cells?

fibroblasts help maintain lung tissue and can release inflammatory mediators when exposed to harmful stimuli

what is MRC-5 useful for studying? (4)

lung cell inflammation

cell injury

cytokine production

protective effects of drugs or natural products

what does LPS stand for?

lipopolysaccharide

what is LPS?

a component of the outer membreane of gram-negative bacteria

what happens when cells detect LPS?

when cells detect LPS inflammatory signalling pathways may become activated and increased inflammatory cytokines

what is LPS used for?

to create an inflammation model

what does an inflammation model do?

helps to compare healthy cells with experimentally inflamed cells and determine whether treatment reduces the response