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different organisms
different microbes have different levels of resistance, endospores are super resistant
number of organisms
more microorganisms require a longer exposure to kill
mode of action means
how well a antimicrobial agent works depending on what part of the microbe it attacks and how it damages it
presence of solvents/organic material
blood, saliva, feces and other substances interfere with antimicrobial activity making it harder to kill and clean bacteria