Unit 2 Part 6 - DNA replication

semi-conservative replication

has a parent and daughter strand

bidirectional

each origin of replication has two replication forks

single bacterial origin of replication

OriC

AT-rich sequences

two hydrogen bonds = easier to separate strands

DnaA box sequences

DnaA protein binds to this inside of the oriC, inside AT-rich sequences to initiate strand separation.

GATC methylation sequences

activation signal for DNA replication

replicon

all the DNA replicated from a single origin

What are the names and functions of the three types of DNA sequences found within OriC?

(1) AT-rich sequences: easy strand separation due to fewer hydrogen bonds.

(2) DnaA box sequences: serve as binding sites for DnaA proteins to initiate strand separation. (

3) GATC methylation sequences: adenine methylation acts as an activation signal for replication. A replicon is all the DNA replicated from a single origin