Culture Media and their Purpose (B&S, Rodriguez)

Alkaline peptone broth

Vibrio

Ashdown medium

Burkholderia pseudomallei

Buffered charcoal-yeast extract (BCYE) agar [w/ antibiotics]

Legionella spp.

Bile esculin agar (BEA)

Group D streptococci and enterococci

Bile esuclin azide agar w/ vancomycin

Vancomycin-resistant enterococci

Bismuth sulfite agar

Salmonella typhi

Blood agar

Determination of hemolytic reactions

Bordet-Gengou (Potato blood glycerol) agar, Regan Lowe agar

Bordetella pertussis

Brilliant green agar

Salmonella spp.

Campy-blood agar, Cefaperazone vancomycin amphotericin agar, Campylobacter thioglycollate broth, Skirrow agar

Campylobacter spp.

CDC anaerobe 5% sheep blood agar

Obligate, slow-growing anaerobe

Cefsulodin-irgasan-novobiocin (CIN) agar

Yersinia spp. and Aeromonas spp.

Cetrimide agar, Seller's agar

Pseudomonas aeruginosa

Charcoal cephalexin blood agar

Bordetella pertussis (PREFERRED)

Chick embryo

Chlamydia and Rickettsia

Chocolate agar

Haemophilus spp. and Neisseria spp.

Chocolate blood agar with horse blood

Haemophilus spp.

Chromogenic media

MRSA and fungi

Columbia colistin-nalidixic acid agar (CNA)

Gram-positive cocci

Cycloserine-cefotixin fructose agar (CCFA)

Clostridium difficile

Cystine blood glucose agar

Francisella tularensis

Cystine-tellurite blood agar, Loeffler's serum medium, Pai medium, Tinsdale agar

Corynebacterium diphtheriae

Czapek's agar

Nocardia asteroides, Aspergillus spp.

Desoxycholate citrate agar

Salmonella and Shigella (stool)

Dieudonne's medium

Vibrio cholerae

Dorset egg medium

Mycobacterium tuberculosis, Nocardia asteroides

Dubos Oleic agar

Mycobacterium tuberculosis

Edward Hayflick agar

Mycoplasma

Ellinghausen, McCullough, Johnson and Harris (EMJH) medium

Leptospira interrogans

Eosin methylene blue (EMB) agar (Levine)

Lactose vs. non-lactose fermenters

Fetal bovine serum agar with vancomycin

Haemophilus ducreyi

Fletcher's semi-solid medium

Leptospira

Foot pads of mice

Mycobacterium leprae

Gram-negative broth (GN)

Enteric pathogens

Hektoen enteric (HE) agar, Xylose Lysine Desoxycholate (XLD) Agar

Salmonella & Shigella vs. other enteric bacilli

Human blood bilayer Tween medium

Gardnerella vaginalis

Kanamycin-Vancomycin blood agar

Bacteroides

Kelly's medium

Borrelia burgdorferi

Litmus milk media

Clostridium spp.

Lombard Dowell medium

Anaerobic organisms

Lowenstein Jensen (LJ) agar, Middlebrook 7H10, Petragnani medium

Mycobacteria

Lysine iron agar

Enterobacteriaceae (deamination/decarboxylation)

MacConkey agar

Lactose vs. non-lactose fermenting enteric bacilli

MacConkey sorbitol agar

E. coli O157:H7

Mannitol salt agar

Staphylococci

Martin Lewis agar, New York City (NYC) agar

Neisseria gonorrheae

McBride medium

Listeria monocytogenes

Methylene blue milk

Enterococci

Mueller Hinton agar

Susceptibility test (antimicrobial testing)

Oxidative-Fermentative (OF) medium

Acinetobacter, Alcaligenes, and Pseudomonas

Phenylethyl alcohol (PEA) agar

Gram-positive cocci & anaerobic Gram-negative bacilli

Polymixin-lysozyme-EDTA-thallous acetate

Bacillus anthracis

Rabbit blood agar with yeast extract

Haemophilus influenzae

Salmonella-Shigella (SS) agar, Tetrathionate broth

Salmonella and Shigella spp.

Schaedler agar

Anaerobes and aerobes

Selenite broth

Salmonella spp.

Streptococcal selective agar (SSA)

Streptococcus pyogenes and S. agalactiae

Thayer-Martin agar

N. gonorrhoeae and N. meningitidis

Thioglycollate broth

Anaerobes, aerobes, microaerophilic & fastidious organisms

Todd-Hewitt broth w/ antibiotics

S. agalactiae in female genital specimens

Trypticase soy broth

Subculturing various bacteria from primary agar plates

Tween 80-albumin broth

Spirochetes