Micro Baltimore Classification- Exam 2

what groups have double stranded DNA

VII and I

what groups have single stranded RNA

VI and IV and V

what groups have single stranded DNA

group II

what groups have double stranded RNA

III

group I genome

dsDNA

group 2 genome

ssDNA

group 3 genome

dsRNA

group IV

ssRNA, positive

group V genome

ssRNA, negaative

group VI genome

ssRNA, DNA intermediate

group VII

dsDNA, RNA intermediate

what does it mean if a virus has an intermediate

it doesn’t go through translation immediately

how is mRNA synthesized

mRNA will always be a 5’  3’ RNA molecule

how is mRNA synthesized in group I

dsDNA is transcribed by host RNA polymerase

how is mRNA synthesized in group II

ssDNA → dsDNA → mRNA using host RNA polymerase

how is mRNA synthesized in group III

dsRNA → mRNA using a viral RNA polymerase (RdRp)

how is mRNA synthesized in group IV

Genome itself = mRNA → it can be translated immediately

how is mRNA synthesized in group V

−ssRNA → +mRNA using a viral RdRp

how is mRNA synthesized in group VI

RNA → DNA → mRNA; reverse transcriptase makes DNA, then host RNA polymerase makes mRNA

how is mRNA synthesized in group VII

DNA → mRNA using host RNA polymerase

What serves as a template for nucleic acid replication in group I

One DNA strand serves as the template for the complementary DNA strand

What serves as a template for nucleic acid replication in group II

The ssDNA is the template to make the complementary DNA strand, creating dsDNA

What serves as a template for nucleic acid replication in group III

One RNA strand serves as the template for the complementary RNA strand

What serves as a template for nucleic acid replication in group IV

The +RNA is the template for making −RNA; then the −RNA serves as the template to make new +RNA

What serves as a template for nucleic acid replication in group V

The −RNA is the template for making +RNA; the +RNA then serves as the template for making new −RNA

What serves as a template for nucleic acid replication in group VI

RNA serves as the template for DNA during reverse transcription; then DNA serves as the template for making new DNA

What serves as a template for nucleic acid replication in group VII

RNA serves as the template for making DNA during reverse transcription

is viral polymerase needed in group I

no, Can use host DNA polymerase and RNA polymerase

is viral polymerase needed in group II

no, Can use host DNA polymerase to make dsDNA, then host RNA polymerase makes mRNA

is viral polymerase needed in group III

yes, Needs a viral RNA-dependent RNA polymerase (RdRp)

is viral polymerase needed in group IV

yes Needs viral RdRp to replicate its RNA genome

is viral polymerase needed in group V

yes, Needs viral RdRp to make +mRNA and replicate its genome

is viral polymerase needed in group VI

yes, Uses reverse transcriptase to convert RNA → DNA; then uses host polymerases

is viral polymerase needed in group VII

Viral polymerase needed for the RNA → DNA step

Uses reverse transcriptase; host polymerases handle the DNA/transcription steps