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Infection Prevention
Methods practiced in healthcare facilities to prevent and control the spread of disease.
Microorganism (Microbe)
A living organism so small that it can only be seen with a microscope.
Pathogens
Harmful microorganisms that invade the body and cause infection or disease.
Nonpathogens
Microorganisms that are part of the normal flora, are beneficial or harmless, and do not cause infection or disease.
Localized Infection
An infection limited to a specific area of the body, causing symptoms near the site.
Systemic Infection
An infection that enters the bloodstream and affects the entire body.
Healthcare-Associated Infection (HAI)
An infection acquired in a healthcare facility while receiving medical care.
Signs of Localized Infection
Pain, redness, swelling, pus, fluid drainage, and heat at the site.
Signs of Systemic Infection
Fever, chills, body aches, nausea, vomiting, weakness, headache, confusion, low blood pressure, or rapid breathing.
Chain of Infection
A six-link model describing how disease spreads from one host to another.
Causative Agent (Chain Link 1)
The disease-causing pathogen, such as bacteria, viruses, fungi, or parasites.
Normal Flora
Microorganisms that live harmlessly in/on the body unless transferred to a different body part.
Reservoir (Chain Link 2)
The warm, dark, moist environment where a pathogen lives and multiplies.
Portal of Exit (Chain Link 3)
Any body opening that allows pathogens to leave an infected person, like the mouth, nose, or open wounds.
Mode of Transmission (Chain Link 4)
How a pathogen travels from host to host via contact, droplets, or air.
Direct Contact Transmission
Spread of germs by physically touching an infected person or their secretions.
Indirect Contact Transmission
Spread of germs by touching a contaminated object, like linens, tissues, or needles.
Portal of Entry (Chain Link 5)
Any body opening on an uninfected person that allows pathogens to enter, such as the nose, mouth, eyes, mucous membranes, or broken skin.
Susceptible Host (Chain Link 6)
An uninfected person who could become ill because their body defenses or immune system are weak.
Aerobic Microorganisms
Microorganisms that require oxygen to live.
Anaerobic Microorganisms
Microorganisms that live and reproduce in the absence of oxygen.
Fomites
Objects contaminated with infectious material that contains pathogens, such as doorknobs, bedpans, urinals, linens, and instruments.
Rickettsiae
Parasitic microorganisms that cannot live outside the cells of another living organism, commonly found in fleas, lice, ticks, and mites.
Communicable Disease
A disease that is transmitted from one person to another.
Bioterrorism
The use of microorganisms or biologic agents as weapons to infect humans, animals, or plants.
Standard Precautions
Infection control rules treating all blood, body fluids, non-intact skin, and mucous membranes as infected for every patient.
Body Fluids
Fluids like blood, saliva, urine, feces, vomit, pus, and semen (excludes sweat).
Transmission-Based Precautions
Extra infection controls added to Standard Precautions based on how a specific disease spreads.
Sharps
Sharp medical objects like needles and razor blades that must be placed in puncture-resistant biohazard containers.
Multidrug-Resistant Organisms (MDROs)
Bacteria resistant to one or more antibiotics commonly used for treatment, like MRSA and VRE.
Airborne Precautions
Controls for airborne germs that float long distances; requires an N95 mask and negative-pressure room.
Droplet Precautions
Controls for germs spread through large droplets traveling up to 6 feet; requires a face mask.
Contact Precautions
Controls for germs spread by direct/indirect touch; requires gloves, gown, isolated room, and dedicated equipment.
Isolate
To keep a patient or object separate from others to prevent infection spread.
Protective (Reverse) Isolation
Isolation methods used to protect vulnerable patients with compromised immune systems from outside pathogens.
Respiratory Hygiene / Cough Etiquette
Infection rules to stop respiratory germs, including covering coughs, disposing tissues, and wearing masks.
Epidemic
A disease outbreak affecting a large number of people in a specific geographic area.
Pandemic
A global disease outbreak that spreads across multiple countries or continents.
Endemic
A disease that is constantly present in a specific region or population at expected levels.
Medical Asepsis (Clean Technique)
Measures used in all healthcare settings to reduce and prevent the spread of pathogens, such as handwashing and wearing clean gloves.
Surgical Asepsis (Sterile Technique)
Sterile technique that makes an object or area completely free of all microorganisms, including spores and viruses, used for invasive procedures.
Sterile Field
An area that is completely free of all microorganisms, established using sterile barriers for surgical or invasive procedures.
Antisepsis
A cleaning process using antiseptics (like alcohol or betadine) to prevent or inhibit pathogen growth on skin; not effective against spores and viruses.
Disinfection
A chemical process that destroys or kills most pathogens on objects or surfaces (not effective against spores); requires a specific wet contact time.
Contact Time
The required amount of time a disinfectant must remain wet on a surface to kill pathogens.
Sterilization
A cleaning process using intense heat or chemicals to destroy all microorganisms, including spores.
Autoclave
A high-pressure steam machine used to sterilize medical instruments.
Ultrasonic / Cavitation
A cleaning process using sound waves through a liquid bath to remove debris from instruments prior to sterilization.
Clean vs. Dirty Objects
Clean means an object has not been contaminated with pathogens; dirty means it has been contaminated with pathogens.
Endogenous Infection
An infection or disease that originates from within the body, such as metabolic disorders or internal bacteria moving to a new body system.
Exogenous Infection
An infection or disease that originates from outside the body, such as invading pathogens, trauma, chemicals, or temperature extremes.
Opportunistic Infection
An infection that occurs when the body's immune system or defenses are weak, which would not normally harm someone with a healthy immune system.
Classes of Microorganisms
The five main groups of microscopic living things: bacteria, viruses, fungi, protozoa, and helminths.
Bacteria Characteristics & Shapes
Single-celled microorganisms that lack nuclei; classified into three main shapes: round (cocci), rod (bacilli), and spiral (spirilla).
Viruses Characteristics
Tiny packages of DNA or RNA encased in protein shells that invade a living host cell, hijack its DNA to replicate, and destroy the host cell.
Fungi Characteristics
Single or multicellular organisms like yeasts and molds that secrete enzymes to break down surrounding cells for nourishment.
Protozoa Characteristics
One-celled animal-like microorganisms often found in decayed materials, animal/bird feces, or contaminated water (causes diseases like malaria).
Helminths Characteristics
Multicellular parasitic organisms commonly called worms or flukes (such as hookworms or pinworms) transmitted via contaminated food or insect bites.
Body Defenses
Natural barriers protecting the body from pathogens, including mucous membranes, cilia, coughing/sneezing, stomach acid, tears, fever, inflammation, and immune response.
WHO 5 Moments for Hand Hygiene
The five key times to clean hands: 1) Before touching a patient, 2) Before clean/aseptic procedures, 3) After body fluid exposure risk, 4) After touching a patient, and 5) After touching patient surroundings.
Handwashing Protocol
Scrub hands and wrists vigorously with soap and water, using friction for at least 20 seconds while keeping fingertips pointed downward.
Alcohol-Based Hand Rub (ABHR) Rules
Preferred method for routine cleaning when hands are not visibly soiled; apply 60-95% alcohol sanitizer and rub hands until completely dry.
Artificial Nails Rule
Artificial nails must not be worn because they harbor bacteria and increase contamination risks, even after frequent handwashing.
PPE Donning Sequence
The correct order to put on PPE: 1) Gown, 2) Mask or Respirator, 3) Goggles or Face Shield, 4) Gloves (gloves go on last and overlap gown cuffs).
PPE Doffing Sequence
The correct order to take off PPE: 1) Gloves, 2) Goggles or Face Shield, 3) Gown, 4) Mask/Respirator (mask comes off last; respirator after leaving room and closing door; wash hands immediately).
Highest Risk Step in PPE Usage
Doffing (removing PPE) is the highest-risk step for self-contamination and must be performed slowly and carefully.
Bloodborne Pathogens Definition
Pathogenic microorganisms found in human blood and certain body fluids that can cause serious infections like HBV, HCV, and HIV.
Hepatitis B (HBV) Details
A severe bloodborne liver virus that can survive in dried blood for at least 7 days; preventable with a free 3-dose vaccine series offered to healthcare workers.
Hepatitis C (HCV) Details
The most common chronic bloodborne infection; can live on surfaces for up to 6 weeks, has no vaccine, but has curative direct-acting antiviral treatments.
Human Immunodeficiency Virus (HIV) & AIDS
A bloodborne virus that attacks the immune system and leads to AIDS; it is fragile outside the human body.
Post-Exposure Prophylaxis (PEP)
Immediate medical evaluation and preventive treatment given after an accidental exposure to blood or body fluids to lower infection risk.
Exposure Control Plan
A written safety plan required by OSHA for healthcare facilities to eliminate or reduce employee exposure to infectious materials.
Engineering Controls
Safety features built into medical devices to reduce workplace hazards, such as needleless IV systems, retractable lancets, and puncture-resistant sharps containers.
Work Practice Controls
Safe behavioral rules that reduce exposure risk, such as washing hands after glove removal and never recapping needles with two hands.
Significant Exposures (OSHA)
Accidental incidents requiring immediate reporting, including needle sticks, cuts from contaminated objects, mucous membrane contact, or non-intact skin contact (including acne).
Hierarchy of Decontamination
The 3-step infection control process: 1) Cleaning (removing organic soil with water/detergent), 2) Disinfection (killing pathogens with chemical wet time), 3) Sterilization (destroying all life and spores using an autoclave).
Clean and Dirty Utility Rooms
Separate facility rooms for clean supplies versus dirty/contaminated items; wash hands before entering clean rooms and before leaving dirty rooms.
Dedicated Equipment in Isolation
Equipment assigned to and used by only one specific patient in isolation (such as a dedicated blood pressure cuff and stethoscope) to prevent cross-contamination.
Handling Soiled Linen
Fold dirty areas inward, keep away from uniform, do not shake, and bag immediately at point of origin.
CRE (Carbapenem-Resistant Enterobacteriaceae)
Antibiotic-resistant intestinal bacteria that can cause severe, life-threatening infections.
Clostridioides difficile (C. diff)
Spore-forming gut bacteria causing severe diarrhea; resistant to alcohol sanitizer and requires soap and water.
OSHA (Occupational Safety and Health Administration)
A federal agency that creates regulations to protect workers from job hazards, including infections.
CDC (Centers for Disease Control and Prevention)
A federal health agency that provides disease prevention guidelines for healthcare workers.
Employer Responsibilities for Infection Control
Responsibilities include establishing an exposure control plan, providing safety education, providing free PPE and hepatitis B vaccines, and keeping a sharps injury log.
Employee Responsibilities for Infection Control
Responsibilities include following Standard Precautions and facility policies, wearing proper PPE, reporting exposures immediately, and taking advantage of the free hepatitis B vacc