Last min to know

glycine

no organic side chain, flexible

proline

at end of helix, start of turns

cysteine

thiol group forms disulfide bonds, binds metals

what are the shape determining residues

proline glycine cysteine

amine formula

NH2

amine/ammonium formula

NH3

carboxyl group formula

O=C-OH

carbonyl group formula

C=O

amide formula

O=C-N-H

hydroxyl group formula

-OH

thiols formula

-SH

sulfide formula

-S-

disulfide formula

-S-S-

whats a globular protein

water soluble globe like proteins w hydrophobic residues

whats a zwitterion

dipolar molecule w pos & neg charge

whats a chaperone

help proteins fold correctly

wat primase do

lays RNA primers on strands so the DNA poly can grab its OH group and start replication

how to recognize H bond acceptor

it has lone pair, no H already attached, NOF

whats translocation

how a growing pep chain in the P site of a ribosome shifts on top of a new amino acid in the A site while the used tRNA in the E site exits

whats nick ligation

sealing okazaki fragments

transcription E requirement

NTP -> NMP + PPi

DNA rep E requirement

dNTP -> dNMP + PPi

translation E requirement

GTP -> GDP + Pi

whats Rho

ends transcription by dislodging RNA poly

whats in RT-PCR dat normal PCR dont have

reverse transcriptase

whats a DNA clamp

hold DNA poly onto template strand