Molecular Exam 3

What genes does RNA Pol I transcribe?

rRNA genes

What genes does RNA Pol II transcribe?

mRNA and small regulatory RNAs

What genes does RNA Pol III transcribe?

tRNA, 5S RNA, snRNA

What genes does RNA Pol IV, V transcribe?

siRNA

What is the sense (+) strand?

DNA coding strand

Non-template strand

What is the antisense (-) strand?

DNA template strand

Non-coding

Most common sigma factor in bacteria

Sigma 70

-35 consensus sequence

TTGACA

-10 consensus sequence

TATAAT

TFIIA function

Stabilizes binding of TBP and TFIIB

TFIIB function

promotor recognition

stabilize early transcribing complex

TFIID function

promotor recognition

DNA bending interacts with regulatory factors

TFIIE function

recruits TFIIH

TFIIF function

Suppresses non-specific DNA binding

captures non-template strand upon melting

TFIIH function

unwinds promoter DNA - Helicase Activity

phosphorylates CTD - Kinase activity

Where does TBP bind? What is a result of this?

Minor groove

Bends DNA

What is BRE?

TFIIB recognition element

Sigma factor job

guide polymerase

housekeeping genes

expressed continuously

developmentally regulated genes