ast-amylase lab

Couple Enzymatic Reaction

Ast and Alt PRINCIPLE

Classic Colorimetric Assay

ALP principle

Lactate Dehydrogenase Reverse Reaction

Ldh principle

Serum Glutamic Oxaloacetic Transaminase

other name AST

Serum Glutamic Pyruvic Transaminase

other name ALT

2611

AST ec number

2612

ALT ec number

3131

ALP ec no

1.1.1.27

Ldh ec no

3.2.1.1

Amylase ec no

Pyridoxal Phosphate

AST and ALT coenzyme/activator

magnesium

ALP coenzyme/actuvator

Nicotinamide Adenine Dinucleotide

LDH coenzyme/actuvator

Calcium, Chloride

Amylase coenzyme/activatir

EDTA plasma

Heparinized Plasma

AST and ALT spx

Unhemolytic serum

Heparinized plasma

ALP spx

Urine

EDTA plasma

Amylase spx

340 nm

AST AND ALT and LDH wavelength

405 nm

ALP AND AMYLASE wavelength

200 ul

AST AND ALT SAMPLE VOL

40 ul

ALP LDH AMYLASE sample vol

1745

AST ALT kinetic factor

2764

ALP kinetic

8095

LDH kinetic

3126

AMYLASE kinetic

INCREASING

ALP AND AMYLASE reaction direction

KARMED METHOD

AST ALT method

Bowers-McComb method

ALP method

wrobleuski-La due method

LDH method

P-nitrophenol phosphate (yellow)

ALP end product

De ritis ratio

ratio between ALT and AST (origin condition of liver disease)

alpha-2-chloro-4-nitrophenyl-beta-1,4-galactopyranosylmaltoside

amylase substrate

alpha-1,4-glycosidic bond

amylose branch

Salivary isoamylase

Pancreatic isoamylase

2 isoenzyme of AMYLASE

Salivary amylase

Most anodal amylase

Pancreatic Amylase

Migrates slower (amylase)

mitochondrial and cytoplasmic ast

Isoenzymes of AST

Continuous monitoring method

Amylase method