BLD 213L microscopy learning objectives

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Last updated 7:48 PM on 9/28/26
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21 Terms

1
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What is the function/purpose of the eyepiece (ocular lens) on a bright field microscope?

2
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What is the function/purpose of the objective lens(es) on a bright field microscope?

3
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What is the function/purpose of the stage on a bright field microscope?

4
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What is the function/purpose of the stage controls on a bright field microscope?

5
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What is the function/purpose of the substage condenser on a bright field microscope?

6
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What is the function/purpose of the aperture diaphragm on a bright field microscope?

7
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What is the function/purpose of the coarse and fine focusing knobs on a bright field microscope?

8
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What is the function/purpose of the field light on a bright field microscope?

9
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<p>What is the magnification on the objective lens?</p>

What is the magnification on the objective lens?

40x

10
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<p>What is the resolution (revolving power) of the objective lens?</p>

What is the resolution (revolving power) of the objective lens?

11
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<p>What is the numerical or working aperture for the objective?</p>

What is the numerical or working aperture for the objective?

12
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<p>What is the working distance and coverslip thickness for this objective?</p>

What is the working distance and coverslip thickness for this objective?

13
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What are three things that should be adjusted on a microscope when increasing or decreasing the lens objective magnification that allow for optimal illumination of the specimen?

14
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What are the 3 main types of objective?

15
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What are the relative expense/quality of the three most common objective types?

16
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What objective type is most commonly used in clinical laboratory microscopes?

17
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How does phase contrast microscopy differ from bright field microscopy and when/why do we use it?

18
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How does polarized microscopy differ from bright field microscopy and when/why do we use it?

19
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How does dark field microscopy differ from bright field microscopy and when/why do we use it?

20
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How does fluorescence microscopy differ from bright field microscopy and when/why do we use it?

21
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How does electron microscopy differ from bright field microscopy and when/why do we use it?