PNB 2264 Lab: Histology

areolar

connective tissue proper, made up of collagen and elastin

hayline cartilage

supportive connective tissue, consists of cells (chondrocytes) lying in groups of two or more

adipose

connective tissue proper, made up of vacuoles containing lipids

cardiac muscle

contains striations and intercalated discs

Hematoxylin

- dark blue staining

- basic/positively charged

- stains acidic/negatively charged substances (ex. DNA/RNA in the nucleus)

Eosin

- pink staining

- acidic/negatively charged

- stains basic/negatively charged substances (ex. proteins in the cytoplasm)

Where is Simple Cuboidal epithelium located?

glands and kidney tubules

Where is Stratified Cuboidal epithelium located?

some glands

Where is Pseudostratified Columnar epithelium located?

respiratory tract

Where is Stratified Squamous epithelium located?

skin and mouth

Where is Stratified Columnar epithelium located?

glandular ducts

Where is Simple Squamous epithelium located?

lungs and blood vessels

Where is Simple Columnar epithelium located?

digestive tract lining

Where is transitional epithelium located?

urinary bladder

basement membrane

Layer between epithelium and underlying connective tissue

a thin, strong sheet of specialized extracellular matrix that anchors epithelial and endothelial tissue to underlying connective tissue and separates different cell layers

simple

single layer of cells

stratified

more than one layer of cells

squamous

flat cells, squished looking

cuboidal

cube shaped, centralized nucleus

columnar

column shaped

Pseudostratified

not all nuclei of cells are at the same level (nuclei not liked up right)

free surface

Part of the epithelial tissue that is exposed to an open area (either the external environment or to the inside of a hollow organ).

dense regular connective tissue

connective tissue proper

Function: attaches muscles to bones or to muscles; attaches bones to bones; withstands great tensile stress when pulling force is applied in one direction

Location: tendons, most ligaments, aponeuroses

lacuna

an empty space or missing portion

osteon

structural unit of compact bone

canaliculi

Hairlike canals that connect lacunae to each other and the central canal

central canal

a fluid-filled channel in the center of the spinal cord

striations

alternate dark and light bands found on skeletal and cardiac muscle

intercalated discs

Attachment sites between the transverse lines between cardiac muscle cells