chymotrypsin reaction mechanism
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Last updated 5:16 PM on 2/19/26
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9 Terms
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1. substrate binding
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2. histidine activates serine for nucleophilic attack
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3. the oxyanion tetrahedral intermediate is stabilized by hydrogen bonds
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4. cleavage of the peptide bond
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5. the covalent acyl-enzyme intermediate
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6. water attacks the carbonyl carbon
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7. second oxyanion tetrahedral intermediate
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8. acid catalysis breaks the acyl-enzyme covalent bond
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9. the product is free to dissociate
