Cell Culture Flashcards

Explain Cell Culture

Removal of cells (animal or plant) and growth in a favourable artificial environment (in vitro)

explain Primary Culture

The stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate.

What does Subcultured / Passaged mean

Transferring cells to a new growth medium to provide more room for continued growth.

How are Cell Lines formed

Passaging or subcultivation of a primary culture

Explain Immortalisation (Cell Culture)

Can be made to proliferate indefinitely given the right treatment, conditions and space.

What is a Cell Strain

Subpopulation of a cell line that has been positively selected from the culture.

What does the Culture Media create

Medium (gel or liquid) to support cellular growth in an artificial environment so cells cultures can survive and proliferate.