Cell Culture Flashcards

Cell Culture

Removal of cells (animal or plant) and growth in a favourable artificial environment (in vitro)

Primary Culture

The stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate.

Subcultured / Passaged

Transferring cells to a new growth medium to provide more room for continued growth.

Cell Line

Passaging or subcultivation of a primary culture begins a cell line

Immortalisation (Cell Culture)

Can be made to proliferate indefinitely given the right treatment, conditions and space.

Cell Strain

Subpopulation of a cell line that has been positively selected from the culture.

Culture Media

Creates the medium (gel or liquid) to support cellular growth in an artificial environment so cells cultures can survive and proliferate.