Cell Lines, CMB, Quiz Bowl

Caco-2 cells originate from which organ and disease?

Colon, colorectal adenocarcinoma

HaCaT cells come from which species?

Human

HK-2 cells originate from which organ?

Kidney

RAW 264.7 cells model which immune cell type?

Macrophages (mouse)

HEK293 cells come from which species?

Human

Are Jurkat cells adherent or suspension?

Suspension (T cells)

What is the species of origin of Jurkat cells?

Human

What is the cell type of NIH/3T3: fibroblast or macrophage?

Fibroblast (NIH/3T3, mouse embryo)

Which cell type on the list is pluripotent and grows in round colonies?

mESCs (mouse embryonic stem cells)

What does “HEK” in HEK293 stand for?

Human Embryonic Kidney

P19 cells come from which species?

Mouse (teratocarcinoma)

RAW 264.7 cells come from which species?

Mouse

HaCaT cells are immortalized cells of which skin cell type?

Keratinocytes

HepG2 cells originate from which organ?

Liver

Vero and COS-7 cells come from the kidney of which animal?

African green monkey

Are HeLa cells adherent or suspension?

Adherent

MCF-7 cells come from which type of cancer?

Breast adenocarcinoma

HCT116 cells come from which tissue and disease?

colon, colorectal carcinoma

Are MCF-7 cells adherent or suspension?

Adherent

Which cell line is famous both for being the first immortalized human line and for its historic role in developing the polio vaccine?

HeLa

Clue 1: I come from an African green monkey kidney.

Vero

Clue 2: I cannot make interferon.

Vero

Clue 3: I was established in Chiba, Japan in 1962

Vero

Clue 1: HPV-18 DNA is integrated in my genome.

HeLa

Clue 2: I grow adherent and double about every 24 hourse.

HeLa

Clue 3: I was the first immortal human cell line, taken from Henrietta Lacks

HeLa

Clue 1: My donor was a 15-year-old boy.

HepG2

Clue 2: I was first called hepatocellular carcinoma, but I am a hepatoblastoma.

HepG2C

Clue 3: I am a liver model with a doubling time of about 48 hours.

HepG2

Clue 1: I was isolated in 1970 from a pleural effusion.

MCF-7

Clue 2: I express estrogen and progesterone receptors.

MCF-7

Clue 3: My name honors the Michigan Cancer Foundation and I am a breast cancer line.

MCF-7

Clue 1: I carry a KRAS G13D mutation.

HCT116

Clue 2: I am mismatch-repair deficient (microsatellite unstable).

HCT116

Clue 3: I am an adherent, epithelial-like human colorectal carcinoma line.

HCT116

Clue 1: I make IL-2 when activated.

Jurkat

Clue 2: I grow in suspension in RPMI-1640.

Jurkat

Clue 3: I was taken from a 14-year-old boy with T-cell leukemia.

Jurkat

Clue 1: CV-1 cells were transformed with origin-defective SV40 to make me.

COS-7

Clue 2: My cells are fibroblast-like.

COS-7

Clue 3: I come from African green monkey kidney.

COS-7

Clue 1: The tumor I came from was induced by Abelson murine leukemia virus.

RAW 264.7

Clue 2: My doubling time is roughly 11-17 hours.

RAW 264.7

Clue 3: I am a mouse macrophage-like line used to study inflammation.

RAW 264.7

Clue 1: Focus-formation assays rely on my strong contact inhibition.

NIH/3T3

Clue 2: I am a fibroblast.

NIH/3T3

Clue 3: I come from a Swiss mouse embryo.

NIH/3T3

Clue 1: I was spontaneously immortalized from the skin of a 62-year-old man.

HaCaT

Clue 2: Extracellular calcium controls my differentation.

HaCaT

Clue 3: I am a human keratinocyte line.

HaCaT

Clue 1: Retinoic acid turns me into neurons.

P19

Clue 2: DMSO can turn me into cardiac and skeletal muscle.

P19

Clue 3: I come from a mouse teratocarcinoma.

P19

Which line forms polarized, enterocyte-like cells with a brush border after ~21 days?

Caco-2

COS-7 was made by transforming CV-1 cells with a defective form of which virus?

SV40

HepG2 is now known to be which disease, from what donor?

Hepatoblastoma, 15-year-old male

Which two hormone receptors does MCF-7 express?

Estrogen Receptor (ER) and the Progesterone Receptor (PR)

Which line is MMR-deficient (MSI) with KRAS G13D?

HCT116

Which viral oncogenes immortalized HK-2?

HPV-16 E6/E7

Which cytokine do activated Jurkat cells produce?

IL-2

Which line came from a tumor induced by Abelson murine leukemia virus?

RAW 264.7

RAW 264.7 doubling time: about 12h, 36h, or 72h?

~12h (11-17h)

HaCaT differentiation is controlled by which extracellular ion?

Calcium

Clue 1: I come from a colon cancer.

Caco-2

Clue 2: After about 21 days I act like small-intestine enterocytes.

Caco-2Cl

Clue 3: I am the standard model for drug absorption assays.

Caco-2

Which two mouse-derived lines here are fibroblast-like or macrophage-like adherent cells?

NIH/3T3 and RAW 264.7

Rank HepG2, Jurkat, and mESCs from shortest to longest doubling time.

mESCs (~12-14h), Jurkat (~24h), HepG2 (~48h)

Which line gives cardiac/skeletal muscle with DMSO and neurons with retinoic acid?

P19

Which additives keep mESCs undifferentiated?

LIF + feeders or 2i

Which line needs ~20% FBS and is used in drug-permeability assays?

Caco-2

MCF-7 was isolated from which body fluid?

Pleural effusion

Why is NIH/3T3 useful in focus-formation assays?

Strong contact inhibition

Which HPV type is integrated in the HeLa genome?

HPV-18

Sudden death: Which line was established in 1962 in Chiba, Japan?

Vero

Clue 1: I come from an embryonic kidney, yet I behave like a neuron.

HEK293

Clue 2: Adenovirus 5 DNA is in my genome.

HEK293

Clue 3: I am a favorite for transfection and making recombinant proteins.

HEK293