Kinetics and Separation / Chromatography

In chromatography, the stationary phase solid while the mobile phase can be______________________ supported on a made of ___________________?

Solid or liquid, liquid

Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2mm thick layer of an adsorbent?

A. Column

B. Gas liquid

C. Paper

D. Thin layer

E. Clear my choice

D. Thin layer

Chromatography cannot be used to purify volatile substances.

True or False

False

The components of the mixture in column chromatography are eluted in order of

A. Increasing polarity and decreasing distribution ratio

B. Decreasing polarity and increasing distribution ratio

C. Decreasing polarity and decreasing distribution ratio

D. Increasing polarity and increasing distribution ratio

D. Increasing polarity and increasing distribution ratio

Characteristics feature of any form of chromatography is the

A. Use of ion exchange resins

B. Use of molecules that is soluble in water

C. Use of a mobile and a stationary phase

D. Calculation of an Rf value for the molecules separated

E. Use of an inert carrier gas

C. Use of a mobile and a stationary phase

Which of the following statements is true for a refractive index detector in HPLC?

A. It can only be used for isocratic elutions

B. It respond to all solutes

C. It is more sensitive than a UV detector

D. It does not respond to many solutes

D. It does not respond to many solutes

Which of the following is/are considered as the carrier gas in gas chromatography?

I. He

II. H₂

III. N2

IV. 02

V. Ar

A. IV

B. 1,11

C. I, II, III, V

D. I, II, III

E. I only

C. I, II, III, V

Which chromatography technique consist of a stationary phase immobilized on a glass plate and an organic solvent acting as a mobile phase?

A. Thin layer chromatography

B. Affinity chromatography

C. Gel exclusion chromatography

D. Planar chromatography

E. lon exchange chromatography

A. Thin layer chromatography

Select the correct statement from the following options.

A. The greater the polarity of solute, more strongly it will be adsorbed on a polar surface.

B. The lesser the polarity of solute, more strongly it will be adsorbed on a polar surface.

C. The greater the polarity of solute, more weakly it will be adsorbed on a polar surface.

D. The greater the polarity of solute, more strongly it will be absorbed on a polar surface.

E. The lesser the polarity of solute, more strongly it will be absorbed on a polar surface.

A. The greater the polarity of solute, more strongly it will be adsorbed on a polar surface.

A hydrophobic compound will preferentially partition into an aqueous solvent.

True or False

False

Chromatogram is

A. Solute concentration vs Elution time

B. Solute concentration vs Elution volume

C. A and B

D. None of the above

C. A and B

The ratio of distance travelled by the sample over the total distance covered by the solvent is referred to as

A. volumetric throughput

B. Retention factor

C. Solubility

D. Miachelis menton constant

B. Retention factor

Which of the following is the disadvantage of helium, which can be used as carrier gas in gas chromatography?

A. Low density

B. Greater flow rate

C. Safe to use

D. Inert gas

E. Expensive

E. Expensive

Which of the following type of chromatography that fits the description given: Description: It is performed with both packed and open tubular columns; it is based on adsorption of gaseous substance on solid surfaces.

A. Planar chromatography

B. Gas-solid chromatography

C. Liquid-liquid chromatography

D. lon exchange chromatography

E. Liquid-solid chromatography

B. Gas-solid chromatography

Which is the best mobile phase for SFC?

A. ethane

B. carbon dioxide

C. carbon monoxide

D. water

E. ammonia

B. carbon dioxide

Which is not generally used for separation of proteins, as proteins get denatured by it?

A. lon-exchange chromatography

B. Chiral chromatography

C. Reverse phase chromatography

D. Affinity chromatography

E. Gel-permeation chromatography

C. Reverse phase chromatography

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme- substrate?

A. lon exchange chromatography

B. High performance liquid chromatography

C. Adsorption chromatography

D. Affinity chromatography

E. Gel-permeation chromatography

D. Affinity chromatography

In reverse phase chromatography, the stationary phase is made

A. either polar or non-polar

B. non-polar

C. Polar

D. neither polar nor non-polar

B. non-polar

Paper Chromatography is a separatory technique that is used to separate

A. metals

B. Complex mixtures

C. Simple mixtures

D. homogeneous mixtures

E. Viscous mixtures

C. Simple mixtures

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme- substrate?

A. lon exchange chromatography

B. High performance liquid chromatography

C. Adsorption chromatography

D. Affinity chromatography

E. Gel-permeation chromatography

D. Affinity chromatography

Chromatography is used to separate

A. molecules

B. Mixtures

C. Inert gases

D. Atoms

B. Mixtures

Chromatography cannot be used to separate delicate products.

True or False

False

For SFC, the mobile phase must exist in this state

A. Critical

B. Liquid

C. Supercritical

D. Gas

E. Gas-liquid

C. Supercritical

In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

A. Liquid chromatography

B. Thin layer chromatography

C. Column chromatography

D. Planar chromatography

E. Gas chromatography

C. Column chromatography

In reversed phase HPLC, there is a

A. non-polar solvent/polar column

B. non-polar solvent/non-polar column

C. polar solvent/polar column

D. polar solvent/non-polar column

D. polar solvent/non-polar column

Chromatography is a physical method that is used to separate and analyze

A. Viscous mixtures

B. Metals

C. Simple mixtures

D. Complex mixtures

E. Organic substance

D. Complex mixtures

Which of the following is/are applications of chromatography?

A. It is used for the analysis of drugs, hormones and vitamins.

B. It is useful for the determination of molecular weights of proteins

C. It is used for the separation of amino acids, proteins and carbohydrates.

D. Helpful for the qualitative and quantitative analysis of complex mixtures.

E. All of these

E. All of these

In which chromatography: stationary phase is more polar than mobile phase?

A. lon exchange chromatography

B. Field flow chromatography

C. Reversed chromatography

D. Normal chromatography

D. Liquid liquid Chromatography

D. Normal chromatography

In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

A. Liquid chromatography

B. Thin layer chromatography

C. Column chromatography

D. Planar chromatography

E. Gas chromatography

C. Column chromatography

The process pf passing a mobile phase through a chromatography column is called which one of the following?

A. Flushing

B. Eluent

C. Washing

D. Partitioning

E. Elution

B. Eluent

Which of the following is NOT a feature of carrier gas used in gas chromatography?

A. It should not be completely pure

B. It should be cheap

C. It must be chemically inert

D. It should be suitable for the detector employed

A. It should not be completely pure

Gas chromatography is used for volatile compounds while HPLC is better for less volatile samples.

True or false

True

Liquid chromatography can be performed in which of the following

ways?

A. Column

B. Liquid surface

C. Planar surfaces

A. B only

B. A or B or C

C. A only

D. A or C

E. A and B

D. A or C

A high molecular weight polymer to which a large number of acidic or basic functional groups have been bonded.

A. lon-exchange extract

B. lon-exchange resin

C. Enzyme inhibitor

D. Enzyme activator

E. Ion polymer

B. lon-exchange resin

lon exchange chromatography is based on the

A. Partition chromatography

B. Electrostatic attraction

C. Adsorption chromatography

D. Electrical mobility of ionic species

B. Electrostatic attraction

Chromatography with solid stationary phase is called

A. Solid chromatography

B. Square chromatograpy

C. Absorption chromatography

D. Circle chromatography

E. Adsorption chromatography

E. Adsorption chromatography

Paper chromatography is

A. Adsorption chromatography

B. Partition chromatography

C. Electrostatic attraction

D. Electrical mobility of ionic species

B. Partition chromatography

Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action?

A. Planar chromatography

B. lon exchange chromatography

C. Column Chromatography

D. Gas chromatography

E. High Pressure Liquid Chromatography

A. Planar chromatography

What is the factor responsible for the separation in column chromatography?

A. Polarity differences between the solvent

B. Polarity indifference between the solvent

C. Polarity differences between the solute

D. Polarity indifference between the solute

C. Polarity differences between the solute

It is a method of separating biochemical mixture based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, protein and nucleic acid.

A. lon exchange chromatography

B. Affinity chromatography

C. Chiral chromatography

D. Affinity or chiral chromatography

E. Size-exclusion chromatography

B. Affinity chromatography

Gas chromatography can be performed in which of the following ways?

A. Column

B. Liquid surface

C. Planar surfaces

A. Column

It is a process for separating components of a mixture in which the different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another.

Chromatography

In Gas-liquid phase chromatography, the stationary phase is composed of ________________ and the mobile phase is liquid made of _________

Liquid, Gas

A combination of paper chromatography and electrophoresis involves

A. Partition chromatography

B. Electrical mobility of the ionic species

C. Electrostatic attraction

D. A and B

D. A and B

It is a graphical representation of separated eluents which can be used to identify compounds and to determine their relative concentrations within the mixture.

Chromatogram

A mobile phase in chromatography that is used to carry solutes or analyte through a stationary phase.

Eluet

In Thin layer chromatography, the stationary phase is made of ______________ and the mobile phase is made of _______________

Solid, Liquid

Liquid chromatography can be performed in which of the following ways?

A. Column

B. Liquid surface

C. Planar surfaces

D. A and C

D. A and C

Which of the following type of

chromatography that fits the description given: Description: It is performed with both packed and open tubular columns; it is based on adsorption of gaseous substance on solid surfaces.

A. Planar chromatography

B. Gas-solid chromatography

C. Liquid-liquid chromatography

D. lon exchange chromatography

E. Liquid-solid chromatography

B. Gas-solid chromatography