Chem 255 Chromatography Techniques

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Last updated 8:50 PM on 10/1/26
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10 Terms

1
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What does SDS do?

It denatures proteins into a linear structure and applies a uniform negative charge, proportional to size.

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SDS

sodium dodecyl sulfate

3
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2-D gel electrophoresis differentiates based on [ ] and then [ ]

charge, size

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2-D gel electrophoresis sorts for charge by

having a pH gradient. proteins will migrate only as far as their isoelectric point.

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agarose has [ ] pores and is used for [ ] electrophoresis

larger, DNA

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polyacrylamide has [ ] pores and is used for [ ] electrophoresis

smaller, protein

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purification based on solubility

centrifugation, salting out

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purification based on size

gel permeation chromatography, SDS-PAGE

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purification based on charge

ion exchange chromatography, gel electrophoresis, isoelectric focusing

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purification based on binding specificity

affinity chromatography