Set 5 — Specified Investigation: Effect of pH on Enzyme Activity

What is the aim of the pH enzyme experiment?

To investigate the effect of pH on enzyme activity

What is the independent variable in the pH experiment?

pH of the reaction mixture

How is pH changed in the experiment?

By using different pH buffer solutions

What is the dependent variable in the pH experiment?

Rate of enzyme activity measured by foam produced per minute

What variable is kept constant in the pH experiment?

Temperature at 25 degrees Celsius

What enzyme source is used in the experiment?

Blended celery filtrate

What is the substrate used in the experiment?

Hydrogen peroxide

Why is washing-up liquid added?

To trap the oxygen produced as visible foam

Why is celery blended?

To release the enzyme from the celery cells

Why is a water bath used?

To maintain a constant temperature

What temperature is used in the pH experiment?

25 degrees Celsius

What is the first step in the pH experiment?

Add pH buffer and washing-up liquid to a graduated cylinder

What is the second step in the pH experiment?

Add blended celery filtrate to the graduated cylinder

What happens after adding celery filtrate?

Place the graduated cylinder in a water bath at 25 degrees Celsius

What is added to start the enzyme reaction?

Hydrogen peroxide

How are results collected in the pH experiment?

Measure foam produced per minute and repeat using different pH buffers