culture techniques

How do we grow microbes?

mimic the conditions where the species are found

what would be a carbon source

sugar the plate uses (glucose, sucrose)

what can you consider for microbe growth?

light, carbon source, amino source, tempature, pH, liquid v solid, oxygen, defined v undefined

what is boiling point?

100 celsius

what is freezing temp?

0 celsius

what is room temp?

20-22 celsius

what is body temp?

30 celsius

what temp are fridges set at?

4 celsius

what are freezers set at?

-20 celsius

what is an ultra deep freezer set at?

-80 celsius

what is an incubator commonly set at?

30 celsius

Agar plate

grow isolated microorganisms

deep agar tube

grow microorganisms that need low oxygen

broth

liquid growth medium that supplies nutrients

slanted agar tube

store cultures for a long-term

why are isolated colonies important?

could be used to start a pure culture

how do you do phenotypic testing on microbes?

grow them in a media that changes color via indicator dye when a certain enzyme is present

strict aerobe

survives on normal oxygen levels

strict anaerobe

no oxygen

aerotolerant anaerobe

no oxygen to grow but can tolerate transient exposure

facultative anaerobe

can tolerate any 02 level

microaerophile

needs a small amount of oxygen

what organisms would you grow if you shake it in a broth jar to grow?

aerobic and facultative anaerobic microorganisms

growing a bacterium that prefers low levels of oxygen and high c02 levels what microbe

microaerophilic using a gas jar and gas pack

defined

all ingredients down to chemical level is known, it allows for faster microbe growth

undefined

ingredients are known but not down to chemical level

example of undefined

yeast, dried/dead cells to provide amino acids, and vitamins, peptone

are extreme oligotrophs possible to grow?

they are difficult to culture due to the extreme conditions they require, peptone, enzymatic digest of meat or veg protein to provide amino acids

when slecting a microbe to extract biofuels from, what is a desirable trait of the microbe?

high lipid levels