Bacteriophages

Bacteriophage steps

1. Entry of phage WHAT

2. WHAT of bacteriophage gene + Degrade host WHAT

3. Assembly of WHAT within host cell

4. WHAT phages

Bacteriophage steps

1. Entry of phage NUCLEIC ACID

2. TRANSCRIPTION of bacteriophage gene + Degrade host CHROMOSOMES

3. Assembly of PHAGES within host cell

4. FREE phages

Bacteriophages form WHAT when they lyse bacteria

Bacteriophages form PLAQUES when they lyse bacteria

Viral traits we can monitor with plaques

• WHAT

• WHAT

Viral traits we can monitor with plaques

• Plaque morphology (size)

• Host range (can it only affect a certain type of bacteria or multiple types of bacteria)

What are the two types of genetic analysis with phages

• Mapping the WHAT with recombination between phages

• Studying linkage and genetic transfer of the BACTERIAL GENOME

What are the two types of genetic analysis with phages

• Mapping the VIRAL GENOME with recombination between phages

• Studying linkage and genetic transfer of the WHAT

Mapping the viral genome

Bacterial DNA exchange uses WHAT

Bacterial DNA exchange uses TRANDUCTION

Transduction

Random incorporation of bacterial DNA into phage head

The WHAT the bacterial genes are in the chromosome, the more likely they are to be WHAT together into a phage head and be WHAT together (WHAT)

The CLOSER the bacterial genes are in the chromosome, the more likely they are to be PACKAGED together into a phage head and be TRANSDUCED together (COTRANSDUCTION)

If you select for two markers and there is a 0% production of the third marker, then the genes are too WHAT to be packed into the phage head together

If you select for two markers and there is a 0% production of the third marker, then the genes are too FAR APART to be packed into the phage head together

The larger the RECOMBINANT FREQUENCY the WHAT the genes

The larger the RECOMBINANT FREQUENCY the FARTHER the genes

The larger the COTRANSDUCTION the WHAT the genes

The larger the COTRANSDUCTION the CLOSER the genes