Enzyme Kinetics

What are the two variables of the Michaelis-Menton Equation?

Substrate concentration and initial velocity

What is the kinetic significance of Km?

Km represents substrate binding affinity (high Km, low affinity)

What is the kinetic significance of Vmax?

Represents max velocity achievable of an enzyme catalyzed reaction

What kind of inhibitor increases Km and does not change Vmax

competitive inhibitor

What kind of inhibitor doesn’t change Km and decreases Vmax

non-competitive inhibitor

What does the X-intercept represent on the line weaver burk plot?

-1/Km

What does the Y intercept represent in the line weaver burk plot?

1/Vmax

what does the slope represent in the lineweaver burk plot

Km/Vmax

Which enzyme class contains dehydrogenases, hydroxylases, and oxidases?

Oxidoreductases

Which enzyme class catalyzes group transfer reactions and contains acyl (HAT), phosphate (kinase) and peptide transfer?

Transferases

Which enzyme class cleaves bonds by addition of a water molecule and includes proteases, and esterase?

Hydrolases

Which enzyme class cleaves by means other than hydrolysis or oxidation and includes decarboxylase?

Lysases

Which enzyme class contains PPIase and PDI, and rearranges existing atoms?

isomerases

Which enzyme class synthesizes C-C, C-S, C-O, C-N bonds in a reaction coupled to the cleavage of high energy phosphate bonds in ATP or other nucleotide, and includes syntheses and ligases?

Ligases