Microbiology 2460 UTA- DR. Parks LAb Practical 1 Study Guide

T/F The level of biosafety is determined by the type(s) of microbes used in a laboratory.

True

The Microbiology Labs at UTA are considered

BSL-2

Biohazard Safety Level 1

are not known to consistently cause disease in healthy adults and present minimal potential hazard to lab workers and the environment. Ex. nonpathogenic strain of Escherichia coli.

Biohazard Safety Level 2

pose moderate hazards to lab workers and the environment.

All requirements for BSL-1 plus:

Access to these labs is restricted to approved personnel only

Labs have self closing doors

Eye wash station(s) are available

An autoclave or equal method of sterilization is available. ex Staphylococcus aureus.

Biohazard Safety Level 3

they can cause serious or potentially lethal disease through respiratory transmission.

All requirements for BSL-2 plus:

Personnel must have necessary vaccinations

Work is perform in a biohazard safety cabinet

Air supply to the lab room must be drawn from a clean supply and cannot be recirculated

Lab room must have two self closing and lockable doors. ex. Mycobacterium tuberculosis,

Biohazard Safety Level 4

are dangerous and exotic, posing a high risk of aerosol-transmitted infections. fatal and without treatment or vaccines

All requirements for BSL-3 plus:

Personnel must change clothing and shower before and after entering these labs.

Biosafety cabinets must be a special level III cabinet.

The laboratory is located in a separate building or in an isolated and restricted zone of the building.

The laboratory has dedicated supply and exhaust air, as well as vacuum lines and decontamination systems. ex Ebola and Marburg viruses.

Anthrax, known as a bioterrorism attacks and is classified as a

Hazard group 3 cause disease, and spread to the community, can cause death

Anthrax is treated by

prophylaxis and is level 2 lab

aseptic technique

involves a combination of protocols that collectively maintain sterility, or asepsis, to prevent contamination of a patient or surface with microbes and infectious agents

failure to practice aseptic technique during many types of clinical procedures can

introduce microbes that can cause death or illness

Medical procedures that carry risk of contamination must be performed in a

sterile field

sterile field

a designated area that is kept free of all vegetative microbes, endospores, and viruses.

T/F experiments and procedures performed in any non-clinical microbiology lab must use aseptic technique to minimize introduction of unwanted microbes

True

Aseptic technique is obtained by creating a sterile field with a

bunsen burner while performing tests

Culturing is the practice

of growing microorganisms, such as yeast or bacteria, in the lab

To create a liquid culture, a colony from an agar plate is added to liquid medium using a

culture loop

The technique used to work aseptically near a

Bunsen burner

T/F light microscopes are the most commonly used microscopes

True

There are two basic types of preparation used to view specimens with a light microscope

wet mounts and fixed specimens.

The simplest type of preparation is the

wet mount

wet mount

the specimen is placed on the slide in a drop of liquid.

wet mount

Solid specimens, such as a skin scraping, can be placed on the slide

fixation

The second (and more commonly used) method of preparing specimens for light microscopy is

"fixing" of a sample refers to

the process of attaching cells to a slide

Fixation is often achieved either by

heating (heat fixing) or chemically treating the specimen

T/F In addition to attaching the specimen to the slide, fixation can also kill microorganisms in the sample, stopping their movement

True

smear

thin layer of the specimen is spread on a slide

the most commonly used type of microscope.

bright-field microscope

bright-field microscope

This is a light microscope as well as a compound microscope

The ocular lenses typically magnify images_____.

10 times (10⨯).

Total Magnification =

Ocular lens magnification X Objective lens magnification

resolution

the ability to tell that two separate points or objects are separate.

Two factors affect resolution:

wavelength and numerical aperture(lens ability to gather light)

numerical aperture

lens's ability to gather light. The higher the numerical aperture, the better the resolution.

total magnification of 1000x)

resolution may be compromised