Biotech EOPA 1 - Intro & Laboratory Practices

Dolly the Sheep

first cloned mammal (1996)

Insluin

first biotechnology (transgenic) produced medicine

Transgenic

term used to refer to an organism that contains genes from other organisms

Genetically Modified Organisms (GMO)

Organisms in which the genetic material (DNA) has been altered in a way that does not occur naturally by mating and/or natural recombination.

Biotech in Agriculture

GMO crops to enhance yield, pest resistance, heat tolerance, etc...

Bioinformatics

application of mathematics and computer science to store, retrieve, and analyze biological data

Precision Medicine

Personal treatment plans based on individual genetics

biopharmaceuticals

use of recombinant DNA technology to develop novel drugs

novel

new; original

PPE - Personal Protective Equipment

a barrier between a person and pathogens; includes gloves, gowns, masks, goggles, and face shields

BSC - Biosafety Cabinets

Use when working with infectious agents, provides sterile working environments and protects you from exposure.

decontamination

The use of physical or chemical means to remove, inactivate, or destroy blood-borne pathogens on a surface or item to the point at which they are no longer capable of transmitting infectious particles and the surface or item is rendered safe for handling, use, or disposal.

10%

Bleach

70%

Alcohol

Radiation

UV light, Microwave

Thermal

Bunsen Burner, Autoclave, boiling water, etc...

MSDS or SDS - (material safety data sheet)

No information on PRICE, but used prepare for appropriate use, storage, and disposal of chemicals.

20µl micropipette

effective tool to aliquot 16µl of solution

Eppendorf tube (1.5ml)

lab equipment works with microcentrifuge

spectrophotometer

instrument that can measure transmitted light and compute the absorption of a solution

High Absorbance & Low Transmittance

A solution with higher concentration

Low Absorbance & High Transmittance

A solution with lower concentration (dilute)

centrifuge

device to separate substances by density by spinning them at high speed

counterbalanced

a term we use with centrifuged to make sure the rotor is balanced by placement of centrifuge tubes.

media or medium

substrates, gel substances, fluids, etc... a substance or environment in which processes occur, organisms grow, or signals travel.

biohazard

medical waste that could cause infection; needles, scalpel blades, blood, body parts

corrosive

having the tendency to erode or eat away, break down, deteriorate, dissolve

poison

a substance that causes illness, injury or death if taken into the body or produced within the body.

Oxidizer

Materials that react chemically with other materials

fire hazard

flammable

Radioactive

emitting or relating to the emission of ionizing radiation or particles.

shock hazard

the term for electrical hazards due to current passing through a human

NFPA - red spot

Fire Hazard

NFPA - Blue Spot

Health Hazard

NFPA - Yellow Spot

Reactivity Hazard

NFPA - White Spot

Specific Hazard

The job of the cell

make proteins

houses DNA

Nucleus

4 locations where DNA can be found

Mitochondria, Chloroplast, Nucleus, Prokaryote Cytoplasm

Instructions for making protein

DNA

simple no nucleus

prokaryotic cell

Complex, yes nucleus

eukaryotic cell