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direction of synthesis
5’ to 3’
read templates
3’ to 5’
exonuclease
breaks phosphodiester bonds, removes primer
polymerase
make phosphodiester bond 5’-3’. fills gaps
DNA helicase
enzyme that breaks hydrogen bonds
primer
short, complementary RNA. Made by a special RNA polymerase (primase)
primase
substrate: rNTP template: DNA
leading strand
Starts at 3’. continuous strand in direction of helicase movement
lagging strand
Discontinuous synthesis starting from multiple primers. synthesize in opposite direction of helicase
DNA polymerase
makes phosphodiester bonds. Substrate 1: dNTP’s 2: template 3: primer: short pre-existing nucleic acid