MMSC270 Lab- Quiz 2 Review

what microbes are we looking to isolate with a nasal swab?

MRSA or MSSA

what media are set up to inoculate a nasal swab?

BAP and MSA

the clinician inoculated the nasal swab and is preparing for observation after twenty four hours of incubation. they first observe the BAP, what finding would lead to further testing on the isolate?

beta hemolysis

the clinical inoculated the nasal swab and is preparing for observation after twenty four hours of incubation. they observe the MSA, what finding would indicate a need for further testing?

MSA fermenter

after gram staining the nasal swab inoculate, the clinician identifies a need for further testing when they identify what result?

gram positive cocci

the clinician identifies a need for further testing on a nasal swab inoculate when they observe that is has a ________ catalase test.

positive

the catalase test is used to identify organisms and differentiate between them. this process involves setting up two QCs with known answers to compare to the isolate being tested. what chemical compound makes up the catalase?

hydrogen peroxide

what physical change indicates a positive catalase test?

bubbling

which antibiotic is used to differentiate between MSSA and MRSA?

methicillin

which antibiotic test is used to differentiate between MSSA and MRSA?

cefoxitin disc diffusion

what is the expected result of the cefoxitin test for MSSA?

>20 mm zone

what is the expected result of the cefoxitin test for MRSA?

<19 mm zone

which media is used to set up a cefoxitin test?

MH

how long after setting up a cefoxitin test will it be read?

18-24 hours

the clinician isolates ______________ from a patients nasal culture and understands that this patient may not respond as well to antibiotic therapy.

MRSA

what microbe are we looking to isolate from a throat culture?

group a streptococcus/strep pyogenes

which patient symptom will prompt the provider to administer a throat swab?

sore throat

which colony morphology on BAP indicates a pathogen may be present in the inoculate of a throat swab?

beta hemolysis

the lab tech isolates a beta hemolytic colony from a throat culture. they identify a need for further testing when they find which gram stain result?

gram positive cocci

which antibiotic resistance test is used to isolate pathogens from throat sample inoculates?

bacitracin

the lab tech expects a _________ catalase test after performing the procedure on suspected strep pyogenes from a throat culture.

negative

what is the expected result of a bacitracin test if group a strep/strep pyogenes is present?

zone of any size

a beta hemolytic gram positive cocci has a negative catalase test. the technician understands the need to set up a bacitracin test, to which they observe a negative result. the tech identifies that this is strep pyogenes and assumes the health care provider will diagnose the patient with which disease?

strep throat

health care providers will collect a sputum culture from deep in the patient's lungs if which disease is suspected?

pneumonia

which microbe is the suspected isolate of a sputum culture of a patient with pneumonia?

streptococcus pneumoniae

which incubator are sputum culture inoculations incubated in?

carbon dioxide

at which temperature do inoculates of body pathogens grow best?

35 degrees C

what does strep pneumoniae look like on a BAP?

alpha hemolysis

the technician isolates an unknown microbe from a sputum sample. they identify the need for further testing when they find which gram stain results?

gram positive cocci

what is the expected result of a streptococcus species catalase test?

negative

what antimicrobial test is set up to differentiate between strep species isolated from sputum cultures?

optichin

the technician understands they have isolated streptococcus pneumoniae from a sputum culture when they observe which results for their optichin test?

>14 mm zone

the technician understands they have isolated viridians streptococcus from a sputum culture when the observe which results from their optichin test?

<13 mm zone

which three dipstick urinalysis findings indicate the need for a urine culture report?

nitrates, leukocytes, protein

which microbe is the most common cause of UTIs?

eschericia coli

which two additional organisms are also likely to cause UTIs if e. coli is not isolated?

klebsiella pneumoniae and pseudomonas aeruginosa

the gram stain of GI/GU pathogens will most likely be what?

gram negative rods

how will e. coli present on BAP and MAC plates?

beta hemolyti;c lactose fermenter

how will klebsiella pneumonia present on BAP and MAC plates?

non-hemolytic mucoid colony; mucoid lactose fermenter

how will pseudomonas aeruginosa present on BAP and MAC plates?

beta hemolytic; non-lactose fermenter

is urine plated using a qualitative or quantitative streaking method?

quantitative

which spot biochems are set up to analyze urine isolates?

oxidase and indole

an oxidase test turns what color when positive?

purple/indigo

an oxidase test turns what color when negative?

light pink/no color change

an indole test turns what color when positive?

teal/turquoise/green

an indole test turns what color when negative?

light pink/no color change