RAID213 Sum 1 Bacteria

L3-L8

Characteristics of Staphylococcus aureus?

Gram ...

In chain/In cluster

Coagulase ...

Gram +

In cluster

Coagulase +

List aerobic gram + in cluster cocci

S. aureus

S. epidermidis

S. sacrophyticus

List beta-hemolytic bacteria

S. pyogenes

S. agalactiae

What bacteria is this?

Aerobic, gram +, cocci, in chain, beta hemolytic grp. A

S. pyogenes

List 2 โรค จาก S. aureus

impetigo

wound infection

celluitis

food poisoning

SSSS

pneumonia/lung abscess

toxic shock

Colours of bacteria after gram stain

Gram + :

Gram - :

purple

red/pink

Gram - appear red after gram stain because it features thin … layer.

The red colour is from counter stain by …

peptidoglycan

safranin

Bacterial cell wall synthesis ใช้อะไรบ้าง

NAM, NAG, amino, BP

Transpeptidase function in bacterial cell wall synthesis

crosslink amino chains

Which enzymes fold circular dsDNA to supercoiled state.

Topoisomerases and gyrases

What gene identify สายพันธุ์ bacteria

16S rRNA

Gram ไหน have thinner peptidoglycan layer and features LPS

Gram -

Gram staining procedure:

1. … จับ peptidoglycan

2. iodine mordant จับแน่น

3. ล้างด้วย alcohol

4. counterstain with …

crystal violet

safranin

What is flesh-eating bacteria?

S. pyogenes

List 2 โรค by S. pyogenes

bacterial pharyngitis

impetigo

erysipelas ผิวแดง

necrotizing fasciitis

scarlet fever

List S. pyogenes sequelae

Post Streptococcal Glomerulonephritis (PSGN)

Rheumatic fever

Rheumatic heart disease

S. agalactiae can cause … and … in adult

neonatal sepsis

acute meningitis or septicemia

What gram + cocci in chain are in Pharyngeal flora?

Viridans

List alpha hemolysis (partial hemolysis) bacteria

S. pneumoniae

Viridans

What is this bacteria?

Irregular stained, gram + bacilli, with chinese letter arrangement

Corynebacterium diphtheriae

List 3 of regular stained aerobic gram + bacilli

Listeria

Lactobacillus

Gardnerella

Bacillus cereus cause … from reheated food

acute diarrhea

Listeria cause …

neonatal septicemia/sepsis

meningitis

Gardnerella cause

bacterial vaginosis

What bacteria has acid fast stain positive result?

Mycobacterium

Nocardia can cause …

subacute pulmonary infection

opportunistic infection in immunocompromised host

What bacteria has positive modified acid fast stain result?

Mycobacterium

Nocardia

S. sacrophyticus cause what?

UTI

What is this bacteria?

Gram + diplococci in lancet shape (with capsule)

S. pneumoniae

What is this bacteria?
Gram + beaded branching filamentous bacilli

Nocardia

What bacteria have mycolic acid?

Mycobacterium

Nocardia

List aerobic gram - diplococci

N. gonorrhoeae

N. meningitidis

M. catarrhalis

Neisseria gonorrhoeae diseases?

urethritis

proctitis

pharyngitis

KEE abbreviations is for what group of Aerobic Gram - bacilli?

lactose fermenter

List all lactose fermenter bacteria

E. coli

Klebsiella

Enterobacter

E. coli diseases?

UTI

enteritis

septicemia

Klebsiella diseases

pneumonia

UTI

liver abscess

septicemia

List non-lactose fermenter group bacteria

Shigella

Salmonella

Proteus

Yersinia pestis

What bacteria group is this?

curved/comma shaped gram - bacilli

Vibrionaceae

List 2 of vibrionaceae bacteria

Vibrio cholerae

Vibrio parahaemolyticus

Vibrio parahaemolyticus cause

seafood-related watery diarrhea

List 2 of non-glucose fermenter bacteria

Pseudomonas aeruginosa

A. baumanni

Burkholderia

Hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP) is associated with what bacteria? (answer 2)

Pseudomonas aeruginosa

A. baumanni

Describe Pseudomonas aeruginosa characteristic

slim bacilli with green pigment

What bacteria is this?

coccobacilli, often in pair

A. baummanni

What bacteria is this?

bipolar stained gram - bacilli

Burkholderia

What bacteria is this?

It is bipolar-stained coccobacilli.

It is associated with cat/dog bite wound infection.

Pasteurella

List 2 of fastidious coccobacilli

H. influenzae

Pasteurella

B. pertussis

H. ducreyi

Brucella

Legionella

What bacteria is associated with goat/sheep?

Brucella

What bacteria is this? (Answer 2)

multi-curved/seagull-winged shaped gram - bacilli

H. pyroli

Campylobacter jejuni

H. pyroli can cause …

peptic ulcer

(may lead to stomach cancer)

Campylobacter jejuni can cause ?

watery/bloody diarrhea

septicemia

Traponema pallidum and Leptospira belong to what group?

Spirochete

What bacteria cause Syphillis?

T. pallidum

Describe characteristics of T. pallidum

gram - cockscrew spirals

Describe characteristics of Leptospira

gram - closed spiral hooked ends

Rodents are associated with the transmission of what bacteria?

Leptospira

Leptospira can cause what disease?

Weil disease

Describe characteristics of Mycoplasma

pleomorphic, no cell wall, membrane has sterols

What is the main method of transmission for Mycoplasme pneumoniae?

droplets

M. pneumoniae cause what disease?

atypical pneumonia

This bacteria is obligate intracellular bacteria. It requires host cell metabolism and lack peptidoglycan layer. What is this bacteria?

Chlamydia

Rickettsia

What is the main mode of transmission for Rickettsia typhi and Orienta tsutsugamushi respectively?

flea, chigger

What bacteria cause endemic (murine) typhus?

Rickettsia typhi

Orienta tsutsugamashi cause what disease?

scrub typhus

List 2 of Mycobacterium

M. tuberculosis

M. leprae

What bacteria is found in armadillo?

M. leprae

Bacteria A targets Schwann’s cell, leading to decreased sensation. What is A?

M. leprae

How M. tuberculosis evade the immune response and replicate?

prevent phagosome lysosome fusion

List all anaerobe bacteria groups (4)

Cutibacterium

Clostidium

Bacteroides

Veillonella

What anaerobe gram +ve bacteria is associated with propionibacterium acne?

Cutibacterium acnes

Gangrene wounds are caused by what bacteria?

Clostidium perfringens

… lead to Clostidium difficile infection

prolonged antibiotics use

Bacteroides fragilis secrete … leading to diarrhea

enterotoxin

How B. fragilis develop resistance against beta-lactam drugs?

secretes beta lactamase