GEL ELECTROPHORESIS

steps in gel electrophoresis

1. make the gel

2. set up the gel apparatus

3. load the DNA sample into the gel

4. hook up the electrical current and run the gel

5. stain the gel and analyze the results

purpose of salt water solution in preparing gel

let the electrical charges flow through the gel

purpose of loading buffer

contains dye for visualization and makes the DNA thicker

short DNA

moves faster, far from the origin

long DNA

moves slower, near the origin

purpose of ethidium bromide

staining solution