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1st step of photosynthesis
plants capture light energy
2nd step of photosynthesis
Combine carbons from CO2 into carbs
3rd step of photosynthesis
Incidentally release O2
reactants for photosynthesis equation
CO2, H20, light
products for photosynthesis equation
C6H12O6, O2, H2O
cellular respiration
reverse process for...
epidermis contains...
stomata
what structure facilitates photosynthesis?
leaves
spongy mesophyll
used for gas exchange
pallisade mesophyll
contains chloroplasts
chlorophyll
pigment within chloroplasts that the plant uses to produce its own energy though photosynthesis
how many membranes do chloroplasts have?
2
lamellae
internal membranes of chloroplasts that are arranged in layers
thylakoids
fused lamellae that form disk-like sacs
grana
columns of thylakoids
stroma
cytoplasm surrounding the grana
where do light-dependent reactions occur?
thylakoids
where do light-independent reactions occur?
thylakoid
phosphorylation
generates ATP by adding phosphate to ADP
what is produced in thylakoids?
NADH, ATP, and release of O2
what is produced in stroma?
carbohydrates
blank
phosphate buffer, distilled water, and chloroplast
used to calibrate the spectrophotometer
experimental and control solution
phosphate buffer, distilled water, chloroplast, and DCPIP
absorbance equation
A = 2-log10 %T
DCIP
artificial electron acceptor that takes the place of NADP+ molecule in light-dependent reactions of photosynthesis
Photosynthesis
chemical process by which light energy is used to convert Co2 and H20 to C6H12O6+O2+H2O
Chloroplast
organelles that contain the pigment chlorophyll
light reaction
Part of photosynthesis that involves light. ATP and NADPH are produced. Takes place on the thylakoid membrane.
Calvin Cycle
light-independent reactions of photosynthesis in which energy from ATP and NADPH is used to build high-energy compounds such as sugar
steps of light reaction
Solar energy to chemical energy
1)light absorbed by chlorophyll
2)energy helps split H2O
3) electron produced from splitting H2O binds to electron acceptor, NADP+
products of Light reaction step
ATP, NADPH, O2
Calvin Cycle steps
CO2 to organic carbon
1)CO2 absorbed from air
2)NADPH gives up electron ATP provides energy
products of Calvin cycle
Sugar, NADP+, ADP+P
wavelength=
color
amplitude=
intensity
Why are chloroplasts green?
they reflect green light
visible light can be
absorbed, reflected, transmitted
chlorophyll pigments absorb
red and blue light
chlorophyll pigments reflect
green light
natural reaction vs artificial reaction
NADP+-->NADPH
oxidized DCPIP-->reduced DCPIP(blue to colorless)
sprectrophotometer
measures the rate of decrease in oxidized DCPIP(measuring absorbance)
absorbance
amount of light(of a specified wavelength) absorbed by a solution)
how to calculate the rate of photosynthesis
slope of absorbance over time
as intensity increases, the rate of photosynthesis
increases
red and blue light will
increase the rate of photosynthesis
experimental
exposed to light and rate of photosynthesis is measured
control
same as experimental, except never exposed to light(covered in aluminum foil)
wavelength range
595-605 nm(wavelength that is absorbed most by the DCPIP)
reaction rate equation
|Δ[A]/Δt/16|
subtract the control rate from the experimental rate to account of external factors