Microscopy Lab Quiz

Parts of Microscope and Functions

Simple Microscope

A microscope that uses one lens

Compound Microscope

A microscope that uses two or more lenses in a line

Stereo (Dissecting) Microscope

Microscope that views objects in 3D

Compound Light Microscope

Microscope that views objects on a slide

Electron Microscope

A microscope that can view very small objects

Magnification

The total value is determined by multiplying the ocular times the objective

Field of View

This is the area seen through oculars

What happens to the field of view with increasing magnification?

decreases

Parcentral

Area in the center of view of the microscope at low power remains centered at high objective

Parfocal

An object in focus at a lower power remains nearly in focus at higher power

Resolution

Distance at which two points can be seen as two separate objects

Depth of Field

Amount of view in focus

How is depth of field adjusted?

Iris diaphragm setting

Why does adjusting the iris diaphragm affect the depth of field?

it adjust the amount of light let through

How are depth of field and resolution related?

They are inversely related

Iris Diaphragm

Part of microscope that controls amount of light. Adjusting this can change depth of field and contrast. Located under the stage.

Condenser Height Adjustment

Part of microscope located under the stage that controls the height of the condenser to focus light on the slide on stage. Adjusting this can change depth of field and contrast.

False

True or False: When setting up microscope start with the highest objective

True

True or Falso: You adjust the oculars to your eye width.

What objective should be near the stage when turning of the microscope

The lowest power