NUCLEIC ACID ISOLATION

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10 Terms

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Nucleic acid isolation

Process of extracting dna or rna from cells or tissues

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Sample preparation

Step 1 of nucleic acid isolation

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Cell lysis

Step 2 of nucleic acid isolation

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Removal of contaminants

Step 3 of nucleic acid isolation

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Sample preparation

Collect a suitable sample;

Preserve DNA/RNA quality;

Prevent contamination

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Cell disruption

Breaks open cells and nuclei;

Releases DNA or RNA;

Uses detergents, enzymes, or mechanical methods

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Purification

Removes proteins, lipids, cellular debris and prevents interference in downstream testing

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Purification

Step 4 of nucleic acid isolation

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Purificaiton

Separate DNA/RNA from remaining impurities;

Increase purity and quality

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Recovery of nucleic acids

Step 5 of nucleic acid isolation