NA2 Simulations

The quality of the analytical results affects directly the quality of the results from the research work

True

How to calculate digestibility

Dig = 100 - [(Mfeed*Nfaeces)/(Mfaeces*Nfeed)]*100
M = marker, N = nutrient

Accuracy and precision

Accuracy

Error with the determinations affects the results found

Error with the determination - accuracy

Overestimation of the marker caused by incomplete hydrolysis of ash residue

Underestimation of the nutrient (nitrogen) caused by leakage of NH3

Recovery

Accuracy is often expressed as recovery

Recovery = measured value/true value *100%

Recovery relates to a systemic erro

Uncertainty within determinations

Uncertainty is the variation within the result caused by the precision of the equipment used

Weighing, analytical balance vs balance

Volume, volumetric flask vs measure cylinder

Effect on uncertainty

Uncertainties of all steps contribute to the total uncertainty in the end result

Smaller uncertainty requires more precise (expensive) equipment and more time

N-simulation steps

Weighing samples

Digestion

Distillation

Titration

Starch simulation - steps

Weighing samples

Extraction soluble sugars

Solubilization starch

Enzymatic degradation starch

Quantification glucose