MLT 227 Blood Products - Storage and Indication for Use

Flashcards covering storage, expiry, yield requirements, and indications for use of various blood products.

What are the storage and shipping temperature requirements for Whole blood?

Storage requirements are $1\text{--}6\text{ deg. C}$, and shipping temperature is $1\text{--}10\text{ deg. C}$.

What is the primary indication for use for Whole blood?

Trauma- Active Bleeding.

1. What is the expiration time for Packed RBCs?

2. What is the special yield requirement for Packed red blood cells prepared with CPD or CP2D?

1. Expiration is $21\text{ days}$

2. The special yield requirement is HcT < 80% or there’s less than 8mg/dL of red blood cells to 10 units of plasma.

What is the expiration period for Packed red blood cells prepared with CPDA-1?

$35\text{ days}$ when stored at $1\text{--}6\text{ deg. C}$.

1. What is the expiration period for Packed RBCs?What is the indication for use for Packed red blood cells with AS-1, -3, -5?

1. Expiration is $42\text{ days}$

2. It is indicated to treat anemia and when Hgb < 7.

What special yield requirements and indications apply to Washed red blood cells?

Wash with Saline and make sure there's no Hemolysis; indicated to remove IgA and to remove WBCs to help stop anaphylaxis shock and ferbrile reactions when RBCs were received (for people with Hgb $< 7$).

What is the expiration time for Washed red blood cells once opened?

$24\text{ hrs.}$ after opening.

What are the storage temperature requirements and expiration period for Frozen red blood cells?

Storage requirement is $\text{<=}-65\text{ deg. C}$ (thaw at $1\text{--}6\text{ deg. C}$), with an expiration of $10\text{ yrs.}$.

What special yield requirement and indications apply to Frozen red blood cells?

Add Glycerol before freezing; indicated for rare donors and family/autologous units.

What is the expiration time and indication for use for Deglycerolized red blood cells?

Expiration is $24\text{ hrs.}$ from thaw; indicated for patients with Hgb $< 7$ and patients with multiple anitbodies.

What are the special yield requirements, dosage, and indication for a Neonatal red cell aliquot?

Yield requirements are Hct $55\text{--}60$, no hemolysis, CMB (-), and Irradiated; dosage is $10\text{mL/Kg}$ for premature babies; indicated when Hgb $< 9\text{--}10$.

What is the required radiation dose and expiration for Irradiated red blood cells?

Radiation dose is $2500\text{cGy}$, and expiration is $28\text{ days}$ or original date if sooner.

What patients are indicated to receive Irradiated red blood cells?

Persons with blood cancers eligible for bone marrow, family donations.

What are the storage requirements and expiration times for Platelets, apheresis?

Stored at $20\text{ deg. -24 deg. C}$ with agitation; expiration is $5\text{ days}$ closed and $4\text{hrs.}$ open.

What are the special yield requirements and platelet count threshold for Platelets, apheresis?

Yield requirements are $>3.0 \times 10^{11}$ Platelets and pH $\text{>=}6.2$; indicated when Plt count $<60 \times 10^3$.

What radiation dose requirement and special matching apply to Platelets, irradiated?

Radiation dose of $2500\text{cGy}$ and HLA matched.

What are the special yield requirements and expiration time for Platelets, pooled?

Yield requirement is $>5.5 \times 10^{10}$ individual Plt; expiration is $4\text{hrs.}$ due to open system.

What are the storage temperature and expiration time for Cryoprecipitate, frozen?

Stored at $-18\text{ deg. C}$ with an expiration of $12\text{ Months}$.

What are the factor levels and indication threshold for Cryoprecipitate?

Factor $8\text{>=}80\text{IU}$ and Fibrinogen $\text{>=}150\text{mg/dL}$; indicated when Fibrinogen $<100\text{mg/dL}$.

What is the expiration time for Cryoprecipitate, single unit thawed?

$6\text{hrs.}$ once thawed.

What are the expiration times for Cryoprecipitate pooled after thawing versus Cryoprecipitate pooled before freezing?

Cryoprecipitate, pooled after thawing expires in $4\text{hrs.}$, whereas Cryoprecipitate, pooled before freezing expires in $6\text{hrs.}$.

What are the storage temperature, expiration time, and indication for Fresh Frozen Plasma?

Stored at $-18\text{ deg. C}$ for $1\text{yr.}$; indicated for replacement of Coagulation Factors, especially $2$, $7$, $9$ and $10$.

What are the storage temperature and expiration time for Fresh Frozen Plasma, thawed versus Thawed Plasma?

Both are stored at $1\text{--}6\text{ deg. C}$; Fresh Frozen Plasma, thawed expires in $24\text{hrs.}$, while Thawed Plasma expires in $5\text{ days}$ from thaw.

What anticoagulant is present in a blood bag?

Na-Citrate.

What preservative is present in a blood bag, and what are the specific functions of Glucose and Adenine?

The preservative is Na-Biphosphate or diphosphate. Glucose is for energy, and Adenine increases ATP and has a preservative effect.